Development and Characterization of a Monoclonal Antibody against Triacetone Triperoxide

• Published in: Bulletin of the Chemical Society of Japan Vol. 86; no. 2; pp. 198 - 202
• Main Authors: Matsumoto, Toru, Yamaguchi, Hiroyasu, Kamijo, Ken’ichi, Akiyoshi, Miyako, Matsunaga, Takehiro, Harada, Akira
• Format: Journal Article
• Language: English
• Published: Tokyo The Chemical Society of Japan 15.02.2013; Chemical Society of Japan
• ISSN: 0009-2673; 1348-0634
• DOI: 10.1246/bcsj.20120253

A monoclonal antibody (mAb) specific to triacetone triperoxide (TATP) was produced from the stable hybridoma cell line 15B-1E, generated by the fusion of myeloma cells with spleen cells isolated from a mouse immunized with a conjugate of 3,3′-(9,12-dimethyl-7,8,10,11,13,14-hexaoxaspiro[5.8]tetradecane-9,12-diyl)dipropanoic acid (DHTDA) with the carrier protein, keyhole limpet hemocyanin. The mAb, 15B-1E, bound DHTDA with a dissociation constant (KD) of 4.7 × 10−7 M. The binding of the mAb to TATP was examined by a competitive indirect enzyme-linked immunosorbent assay (ciELISA) and a surface plasmon resonance (SPR) assay. The KD value of mAb 15B–1E to TATP was 5.2 × 10−5 M. Using an mAb raised against DHTDA, we found that 1.8 µM TATP could be detected using mAb 15B–1E.