Isolation of Turkey Adrenocortical Cell Angiotensin II (AII) Receptor Partial cDNA: Evidence for a Single-Copy Gene Expressed Predominantly in the Adrenal Gland

A turkey adrenocortical cell All receptor cDNA fragment (714 bp) was isolated by RT-PCR using oligonucleotide primers based on rat aortic smooth muscle (RASM) and bovine adrenal type-I (AT1) receptor cDNA coding sequences as primers. Sequence analysis indicated 73% nucleotide identity and 78% ammo a...

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Published inBiochemical and biophysical research communications Vol. 191; no. 3; pp. 1073 - 1080
Main Authors Carsia, R.V., Mcilroy, P.J., Kowalski, K.I., Tilly, J.L.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 31.03.1993
Elsevier
Subjects
Adrenal Glands - metabolism
Amino Acid Sequence
Angiotensin II - metabolism
Animals
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Receptors, Angiotensin - genetics
Receptors, Angiotensin - metabolism
Restriction Mapping
RNA, Messenger - genetics
Turkeys
Adrenal glands
Nucleotide sequence
Copy number
Goose
Peptide hormone
Tissue specificity
Gene expression
Vertebrata
Complementary DNA
Gene
Aves
Angiotensin II
Hormonal receptor
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Summary:A turkey adrenocortical cell All receptor cDNA fragment (714 bp) was isolated by RT-PCR using oligonucleotide primers based on rat aortic smooth muscle (RASM) and bovine adrenal type-I (AT1) receptor cDNA coding sequences as primers. Sequence analysis indicated 73% nucleotide identity and 78% ammo acid identity to the RASM AT1 receptor. Notable differences were 1) two additional Cys at positions 92 and 99 (first extracellular loop), 2) deletion of amino acid 168, formation of a triplet Asn sequence (Asn186,187,188) and substitution of Arg192 with Pro (second extracellular loop) and 3) two additional potential protein kinase C phosphorylation sites, Thr221 and Thr233 (third intracellular loop). Southern blot analysis indicated that the receptor is a product of a single-copy gene. Northern blot analysis indicated at least three mRNA transcripts (7.5, 3.5 and 2.0 kb) expressed predominantly in the adrenal gland.
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1993.1326