Inhibition of planktonic and sessile Salmonella enterica cells by combinations of enterocin AS-48, polymyxin B and biocides
Enterocin AS-48 was tested against planktonic Salmonella enterica UJ3197 in combination with polymyxin B and/or biocides. Enterocin AS-48 added at 50 mg/l or 100 mg/l in combination with 2 mg/l polymyxin B caused partial or complete growth inhibition of S. enterica UJ3197 at 24 h incubation. At 2 mg...
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Published in | Food control Vol. 30; no. 1; pp. 214 - 221 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Kidlington
Elsevier Ltd
01.03.2013
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Enterocin AS-48 was tested against planktonic Salmonella enterica UJ3197 in combination with polymyxin B and/or biocides. Enterocin AS-48 added at 50 mg/l or 100 mg/l in combination with 2 mg/l polymyxin B caused partial or complete growth inhibition of S. enterica UJ3197 at 24 h incubation. At 2 mg/l, polymyxin B did not enhance significantly the inhibitory effect of biocides against strain UJ3197, except for 25 mg/l cetrimide. The combination of polymyxin B (2 mg/l) and enterocin AS-48 (25 mg/l) significantly inhibited growth of strain UJ3197 in combination with 2.5 or 25 mg/l cetrimide, 25 mg/l hexadecylpyridinium chloride, 500 mg/l chlorhexidine, and also with 0.16 or 1.6 mg/l poly-(hexamethylen guanidinium) hydrochloride. Biofilms formed by four Salmonella strains (S62, S64, UJ3197, UJ3198) on polystyrene microtiter plates were treated with biocides singly or in combinations with polymyxin B (2 mg/l), enterocin AS-48 (25 or 50 mg/l) or both. Sessile salmonellae showed an increased tolerance to all biocides tested compared to planktonic cells. At the concentration tested, polymyxin B did not enhance the bactericidal activity for any of the biocides on sessile salmonellae. Enterocin AS-48 improved the activity of most biocides, but only for some of the strains and only for the highest bacteriocin concentration tested in most cases. The combinations of enterocin AS-48 and polymyxin B improved inactivation of sessile salmonellae for all biocides tested, although the degree of inactivation was highly dependent on strain and biocide. The combination of polymyxin B (2 mg/l) and enterocin AS-48 (50 mg/l) improved significantly (p < 0.05) the inactivation of all Salmonella strains in sessile state by the biocides benzalkonium chloride, cetrimide, triclosan, hexachlorophene, chlorhexidine, poly-(hexamethylen guanidinium) hydrochloride, and P3-oxonia.
► Polymyxin B potentiates growth inhibition of Salmonella enterica by enterocin AS-48. ► A combination of polymyxin B and enterocin AS-48 improves the inhibition of planktonic Salmonella by biocides. ► Sessile S. enterica is much more resistant to biocides than planktonic cells. ► A combination of polymyxin B and enterocin AS-48 improves inactivation of S. enterica by selected biocides. |
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Bibliography: | http://dx.doi.org/10.1016/j.foodcont.2012.07.011 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0956-7135 1873-7129 |
DOI: | 10.1016/j.foodcont.2012.07.011 |