Phosphorus dendrimers and photodynamic therapy. Spectroscopic studies on two dendrimer-photosensitizer complexes: Cationic phosphorus dendrimer with rose bengal and anionic phosphorus dendrimer with methylene blue

• Published in: International journal of pharmaceutics Vol. 492; no. 1-2; pp. 266 - 274
• Main Authors: Dabrzalska, Monika, Zablocka, Maria, Mignani, Serge, Majoral, Jean Pierre, Klajnert-Maculewicz, Barbara
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2015; Elsevier
• Subjects: Chemical Sciences; Coordination chemistry; Dendrimers - chemistry; Drug delivery system; Methylene blue; Methylene Blue - chemistry; Phosphorus - chemistry; Phosphorus dendrimer; Photochemotherapy; Photodynamic therapy; Photosensitizer; Photosensitizing Agents - chemistry; Rose bengal; Rose Bengal - chemistry; Spectrophotometry, Ultraviolet; PDT; PS; Photodynamic therapy; PPI; Phosphorus dendrimer; PpIX; Rose bengal; PAMAM; Methylene blue; RB; 1cat; PEG; MB; ALA; ROS; Photosensitizer; Drug delivery system; 1an
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2015.06.014

[Display omitted] Dendrimers due to their unique architecture may play an important role in drug delivery systems including chemotherapy, gene therapy and recently, photodynamic therapy as well. We investigated two dendrimer-photosensitizer systems in context of potential use of these systems in photodynamic therapy. The mixtures of an anionic phosphorus dendrimer of the second generation and methylene blue were studied by UV–vis spectroscopy while that of a cationic phosphorus dendrimer (third generation) and rose bengal were investigated by spectrofluorimetric methods. Spectroscopic analysis of these two systems revealed the formation of dendrimer-photosensitizer complexes via electrostatic interactions as well as π stacking. The stoichiometry of the rose bengal-cationic dendrimer complex was estimated to be 7:1 and 9:1 for the methylene blue-anionic dendrimer complex. The results suggest that these polyanionic or polycationic phosphorus dendrimers can be promising candidates as carriers in photodynamic therapy.