Heterologous expression of a polyketide synthase ACRTS2 in Aspergillus oryzae produces host-selective ACR toxins: coproduction of minor metabolites

• Published in: Bioscience, biotechnology, and biochemistry Vol. 86; no. 3; pp. 287 - 293
• Main Authors: Kotani, Akari, Ozaki, Taro, Takino, Junya, Mochizuki, Susumu, Akimitsu, Kazuya, Minami, Atsushi, Oikawa, Hideaki
• Format: Journal Article
• Language: English
• Published: England 24.02.2022
• Subjects: Aspergillus oryzae - enzymology; Aspergillus oryzae - genetics; Aspergillus oryzae - metabolism; Gene Expression; Mycotoxins - biosynthesis; Mycotoxins - genetics; Mycotoxins - metabolism; Polyketide Synthases - genetics; Polyketide Synthases - metabolism; heterologous expression; Aspergillus oryzae; fungal metabolites; ACR toxin; host-selective toxin
• ISSN: 1347-6947; 1347-6947
• DOI: 10.1093/bbb/zbab214

Previously, we succeeded to produce the core structure of the host-selective ACR toxin (1) on brown leaf spot on rough lemon when the polyketide synthase ACRTS2 gene was heterologously expressed in Aspergillus oryzae (AO). To confirm the production of 1 in AO, the detection limit and suppressing decarboxylation were improved, and these efforts led us to conclude the direct production of 1 instead of its decarboxylation product. During this examination, minor ACR-toxin-related metabolites were found. Their structure determination enabled us to propose a decarboxylation mechanism and a novel branching route forming byproducts from the coupling of the dihydropyrone moiety of 1 with the acetaldehyde and kojic acid abundant in AO. The involvement of putative cyclase ACRTS3 in the chain release of linear polyketide was excluded by the coexpression analysis of ACRTS2 and ACRTS3. Taken together, we concluded that the production of 1 in AO is solely responsible for ACRTS2.