308G>A and –1031T>C tumor necrosis factor gene polymorphisms in Tunisian patients with coronary artery disease

Background: Recent research has shown that inflammation plays a key role in coronary artery disease (CAD) and other manifestations of atherosclerosis. Several lines of evidence support a key role for tumor necrosis factor-α (TNF-α), a potent immunomodulator and pro-inflammatory cytokine, in the deve...

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Published inClinical chemistry and laboratory medicine Vol. 47; no. 10; pp. 1247 - 1251
Main Authors Ghazouani, Lakhdar, Khalifa, Sonia-Ben-Hadj, Abboud, Nesrine, Addad, Faouzi, Khalfallah, Ali Ben, Brahim, Nsiri, Mediouni, Mounira, Almawi, Wassim Y., Mahjoub, Touhami
Format Journal Article
LanguageEnglish
Published Berlin Walter de Gruyter 01.10.2009
De Gruyter
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Summary:Background: Recent research has shown that inflammation plays a key role in coronary artery disease (CAD) and other manifestations of atherosclerosis. Several lines of evidence support a key role for tumor necrosis factor-α (TNF-α), a potent immunomodulator and pro-inflammatory cytokine, in the development of atherosclerosis and in complications of CAD. Methods: We investigated the possible association between CAD and the TNF gene promoter polymorphisms –308G>A and –1031T>C in a Tunisian population. We compared the distribution of these polymorphisms between 418 patients with CAD and 406 healthy controls using polymerase chain reaction restriction fragment length-polymorphism analysis. Results: The frequency of the TNF-α –308A allele in the control group was similar to that observed in CAD patients [p=0.78; odds ratio (OR)=1.15; 95% confidence interval (CI)=0.86–1.55], but higher than those described in other Europeans, such as in the French, Finnish and Spanish. Concerning the TNF-α –1031T/C polymorphism, the same distribution was observed between patients with CAD and controls (p=0.12; OR=1.27; 95% CI=0.94–1.72). In addition, the genotype and allele frequencies of control individuals were comparable to those previously reported in healthy Tunisian controls and other ethnic groups. Haplotype analysis (TNF-α –308G>A and –1031T>C) demonstrated no significant association between TNF haplotypes and CAD. Conclusions: We conclude that TNF promoter gene polymorphisms at position –308G>A and –1031T>C do not play a major role in the pathogenesis of CAD in the Tunisian population. Clin Chem Lab Med 2009;47:1247–51.
Bibliography:istex:E9FC2D69BE25112750E91423006A3EE55D2BC5C7
ark:/67375/QT4-SG62BQ9K-2
ArticleID:cclm.2009.287
cclm.2009.287.pdf
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ISSN:1434-6621
1437-4331
DOI:10.1515/CCLM.2009.287