The regulation of the biosynthesis of glutathione in leaves of barley ( Hordeum vulgare L.)

• Published in: Plant science (Limerick) Vol. 41; no. 1; pp. 11 - 17
• Main Authors: Smith, I.K., Kendall, A.C., Keys, A.J., Turner, J.C., Lea, P.J.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.10.1985; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; catalase deficient mutant; Economic plant physiology; Fundamental and applied biological sciences. Psychology; glutathione; Hordeum vulgare L; localisation; Metabolism; Metabolism. Physicochemical requirements; Nitrogen metabolism and other ones (excepting carbon metabolism); Nutrition. Photosynthesis. Respiration. Metabolism; peroxide; Plant physiology and development; regulation of biosynthesis; regulation of biosynthesis; peroxide; GSSG; CDT; TCA; glutathione; Chl; localisation; Hordeum vulgare L; GSH; catalase deficient mutant; Monocotyledones; Catalase; Hordeum vulgare; Gramineae; Enzyme; Angiospermae; Spermatophyta; Biosynthesis; Plant leaf; Peroxides; Cereal crop; Glutathione
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/0168-9452(85)90059-7

Between 50 and 65% of the glutathione in barley leaves was present in the chloroplasts depending upon the light regime. However, only 66–76% of the chloroplast glutathione was present in the reduced state (GSH) as opposed to 97–98% of that in the cytoplasm. In shoots treated with the catalase inhibitor aminotriazole and in shoots of the catalase deficient barley mutant RPr 79/4 exposed to air, the glutathione level increased 3-fold in 8 h in the light. The increase was accounted for by a rise in both the chloroplast and cytoplasm level of oxidised glutathione (GSSG), the GSH concentration remained relatively constant in both compartments. Only 2–3% of applied 35SO 4 was metabolised to glutathione by wild-type shoots. In aminotriazole-treated plants this value rose to 17.9% and in the mutant RPr 79/4 exposed to air to 32%.