Cellular reactive oxygen species inhibitory constituents of Hypericum thasium Griseb

• Published in: Phytochemistry (Oxford) Vol. 70; no. 2; pp. 244 - 249
• Main Authors: Demirkiran, Ozlem, Ahmed Mesaik, M., Beynek, Hayrettin, Abbaskhan, Ahmed, Iqbal Choudhary, M.
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 2009; Elsevier
• Subjects: Benzophenone glycosides; Biochemistry & Molecular Biology; Biological and medical sciences; Chemical constitution; Fundamental and applied biological sciences. Psychology; General pharmacology; Guttiferae; Hypericum - chemistry; Hypericum thasium; Inhibition of ROS; Inhibitory Concentration 50; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Medical sciences; Molecular Structure; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant physiology and development; Plant Sciences; Reactive oxygen species (ROS); Reactive Oxygen Species - chemistry; Science & Technology; Benzophenone glycosides; Hypericum thasium; Guttiferae; Inhibition of ROS; Reactive oxygen species (ROS); GLYCOSIDES; CHEMI-LUMINESCENCE; CHEMILUMINESCENCE; FLAVONOIDS; XANTHONES; ANTIOXIDANT; BENZOPHENONE; ASSIGNMENT; C-13 NMR; DERIVATIVES; Hypericum perforatum; Benzophenone derivatives; Reactive oxygen species; Weed; Pharmacognosy; Chemical structure; In vitro; Medicinal plant; Dicotyledones; Angiospermae; Plant origin; Spermatophyta; Glycoside; Inhibition
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2008.10.006

Four benzophenone derivatives 1–4, a known benzophenone 5 and four known flavonoids were isolated from EtOAc extract of Hypericum thasium. Lucigenin- and luminal-based chemiluminescence assays were employed to monitor the inhibitory activity of these compounds against reactive oxygen species produced by human polymorphoneutrophils. The phytochemical investigation of the ethyl acetate extract of Hypericum thasium has led to the characterization of four benzophenone derivatives 1–4, a known benzophenone 5 and four known flavonoids, quercetin ( 6), quercitrin ( 7), isoquercetin ( 8), and 3, 8′′-biapigenin ( 9). Lucigenin- and luminal-based chemiluminescence assays were employed to monitor the inhibitory activity of these compounds towards the production of reactive oxygen species (ROS) by human polymorphoneutrophils (PMNs). The assay results showed that benzophenones 1 and 3 are extracellular inhibitors of ROS production, while flavonoids 6, 8, and 9 can modulate intracellular ROS production.