Functional degradation of myelin basic protein. The proteomic approach

• Published in: Bioorganicheskaia khimiia Vol. 37; no. 1; pp. 39 - 47
• Main Authors: Bacheva, A. V, Belogurov, A. A, Kuzina, E. S, Serebryakova, M. V, Ponomarenko, N. A, Knorre, V. D, Govorun, V. M, Gabibov, A. G
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 2011; SP MAIK Nauka/Interperiodica
• Subjects: Amino Acid Sequence; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Calpain - metabolism; Cathepsin D - metabolism; Cell Line; Cricetinae; Cricetulus; experimental autoimmune encephalomyelitis; Humans; Life Sciences; mass spectrometry; Matrix Metalloproteinase 3 - metabolism; Matrix Metalloproteinase 9 - metabolism; Mice; Mice, Inbred BALB C; Molecular Sequence Data; myelin basic protein; Myelin Basic Protein - metabolism; Organic Chemistry; Peptide Fragments - metabolism; Peptide Hydrolases - metabolism; proteases associated with multiple sclerosis; proteasome endopeptidase complex; Proteasome Endopeptidase Complex - metabolism; Proteomics; Trypsin - metabolism; proteasome; mass spectrometry; proteases associated with multiple sclerosis; experimental autoimmune encephalomyelitis; myelin basic protein
• ISSN: 1068-1620; 0132-3423; 1608-330X
• DOI: 10.1134/S1068162011010031

Proteolytic degradation of autoantigens is of prime importance in current biochemistry and immunology. The fundamental issue is the functional role of peptides produced in the process of change of the hydrolysis specificity during the transition from the normal to a pathologic state. In some cases identification of specific peptide fragments can be a diagnostic and prognostic criterion of the pathology progress. The subject of this work is the comparative study of degradation peculiarities of one of the major neuroantigens, myelin basic protein, by proteases activated upon the development of a pathological demyelinating process, and by proteasomes of different origin. Comparison of the specificity of the tested biocatalysts in some cases demonstrated critical changes in the set of myelin basic protein fragments capable of being presented on the major histocompatibility complex class I upon neurodegeneration, which may promote the development of autoimmune pathological processes.