Preliminary Evaluation of BMP-2 Expression and Histological Characteristics During Apexification with Calcium Hydroxide and Mineral Trioxide Aggregate

Histological characteristics and bone morphogenetic protein-2 (BMP-2) expression were evaluated in apexification procedures using calcium hydroxide [Ca (OH) 2] and mineral trioxide aggregate (MTA). In three of four quadrants of the mouth, monkey ( Macaca fascicularis) teeth were infected by pulpal e...

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Published inJournal of endodontics Vol. 31; no. 4; pp. 275 - 279
Main Authors Ham, Karla A., Witherspoon, David E., Gutmann, James L., Ravindranath, S., Chooi Gait, Toh, Opperman, Lynne A.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.2005
Subjects
Aluminum Compounds - pharmacology
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins - biosynthesis
Calcium Compounds - pharmacology
Calcium Hydroxide - pharmacology
Dental Pulp Necrosis - therapy
Drug Combinations
Macaca fascicularis
Oxides - pharmacology
Periodontium - metabolism
Regeneration - drug effects
Root Canal Filling Materials - pharmacology
Root Canal Obturation - methods
Silicates - pharmacology
Tooth Apex - drug effects
Tooth Apex - physiology
Transforming Growth Factor beta - biosynthesis
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Summary:Histological characteristics and bone morphogenetic protein-2 (BMP-2) expression were evaluated in apexification procedures using calcium hydroxide [Ca (OH) 2] and mineral trioxide aggregate (MTA). In three of four quadrants of the mouth, monkey ( Macaca fascicularis) teeth were infected by pulpal exposure to saliva. After 90 days, each group was treated with Ca (OH) 2 or MTA. Infected, untreated teeth were used as negative controls and normally developing teeth were used as positive controls. Areas within the apical region of each sample were scored based upon histological findings and BMP-2 expression. Overall, BMP-2 expression in all areas was similar in the Ca (OH) 2, MTA, and infection/no treatment groups, which tended to be more immunoreactive for BMP-2 than the untreated group. Use of MTA in combination with Ca (OH) 2 may initiate regeneration of the periodontium more quickly than either material used alone, but further studies involving MTA use in apical closure are indicated.
ISSN:0099-2399
1878-3554
DOI:10.1097/01.don.0000140584.65320.cc