Quantitation of Platelet-Associated IgG by Radial Immunodiffusion

Platelet-associated IgG (PAIgG) was measured by a simple radial immunodiffusion technique using washed solubilized platelets and commercially available immunoplates. Subjects with normal platelet counts had PAIgG levels of 1.5–7.0 fg/platelet. Subjects with idiopathic immune thrombocytopenic purpura...

Full description

Saved in:
Bibliographic Details
Published inBlood Vol. 57; no. 4; pp. 809 - 811
Main Authors Morse, Bernard S., Giuliani, Dennis, Nussbaum, Murray
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.1981
Subjects
Blood Platelets - immunology
Citrates - pharmacology
Edetic Acid - pharmacology
Glucose - pharmacology
Humans
Immunodiffusion - methods
Immunoglobulin G
Platelet Count
Thrombocytopenia - immunology
Online AccessGet full text

Cover

More Information
Summary:Platelet-associated IgG (PAIgG) was measured by a simple radial immunodiffusion technique using washed solubilized platelets and commercially available immunoplates. Subjects with normal platelet counts had PAIgG levels of 1.5–7.0 fg/platelet. Subjects with idiopathic immune thrombocytopenic purpura (ITP) had levels ranging from 5.7 to 70.5 fg/platelet. All patients with recurrent ITP and 85% of patients with acute ITP had elevated PAIgG. Elevated PAIgG was also found in 17% of patients with recovered ITP, 40% of patients with SLE and thrombocytopenia, 57% of patients with thrombocytopenia occurring during the course of septicemia, and 100% of patients with IgG myeloma in whom the serum IgG level was clearly elevated, regardless of the platelet count. The results are similar to reports that used more complex techniques.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V57.4.809.809