The Effects of cAMP on the Expression of Glycolytic Isozymes in Activated Peripheral Human T Lymphocytes

• Published in: Archives of biochemistry and biophysics Vol. 302; no. 2; pp. 398 - 401
• Main Authors: Marjanovic, S., Wollberg, P., Skog, S., Heiden, T., Nelson, B.D.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.1993; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cell Cycle - drug effects; Colforsin - pharmacology; Cyclic AMP - pharmacology; Enzyme Induction; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Glycolysis - drug effects; Glycolysis - physiology; Humans; Isoenzymes - biosynthesis; Isoenzymes - drug effects; L-Lactate Dehydrogenase - biosynthesis; L-Lactate Dehydrogenase - drug effects; L-Lactate Dehydrogenase - genetics; Lymphocyte Activation - drug effects; Lymphocyte Activation - physiology; Oxidoreductases; Phosphopyruvate Hydratase - biosynthesis; Phosphopyruvate Hydratase - drug effects; Pyruvate Kinase - biosynthesis; Pyruvate Kinase - drug effects; RNA, Messenger - analysis; T-Lymphocytes - drug effects; T-Lymphocytes - enzymology; Human; Enzyme; Isozyme; Cell cycle; Forskolin; T-Lymphocyte; Cyclic AMP; Lactate dehydrogenase; Activation; Inhibition
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1006/abbi.1993.1230

Forskolin, an activator of adenylate cyclase, inhibits mitogen induction of glycolytic isozymes in human peripheral T cells. Inhibition of lactate dehydrogenase isozyme activity is correlated with lowered mRNA levels, suggesting a transcriptional block in the presence of increased cAMP. Forskolin added with the mitogen, or 12-24 h after the mitogen, strongly inhibits isozyme expression and DNA synthesis, and causes cells to accumulate in the GO or early Gi phase of the cell cycle. The data suggest that DNA synthesis and isozyme expression are both inhibited by a cAMP-sensitive step(s) in the early activation or progression phase.