Rapid detection of trace malachite green using a fluorescence probe based on signal amplification through electrostatic self-assembly of CdTe QDs and polystyrene microsphere

• Published in: Marine pollution bulletin Vol. 151; p. 110812
• Main Authors: Yang, Ji, Lin, Zheng-Zhong, Huang, Zhi-Yong
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2020; Elsevier BV
• Subjects: Amplification; Animals; Aquaculture; Cadmium Compounds; Cadmium tellurides; Detection; Environmental Monitoring - methods; Fish; Fish culture; Fluorescence; Fluorescence in situ hybridization; Fluorescent indicators; Malachite green; Microspheres; Models, Chemical; Molecular Imprinting; Molecularly imprinted film; Polystyrene; Polystyrene resins; Polystyrenes - chemistry; Quantum Dots; Rosaniline Dyes - analysis; Self-assembly; Signal amplification; Spectrometry, Fluorescence; Tellurium; Molecularly imprinted film; Polystyrene; Quantum dots; Signal amplification; Malachite green
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2019.110812

A fluorescence probe was delicately designed for the detection of malachite green (MG) in water and fish samples. Through the electrostatic self-assembly of CdTe QDs on the surface of polystyrene (PS) microspheres, the fluorescence signal was amplified. After grafting molecularly imprinted film, the fluorescence probe of MIP@PS@CdTe was fabricated and applied to the detection of MG based on fluorescence quenching. The linear range of MG detection was 0.01–20 μmol L−1, and the detection limit was 4.7 nmol L−1 (3σ, n = 9) which was much lower than those of the previous reports. The recoveries of MG in aquaculture water and fish samples ranging from 87.6% to 105.4% illustrated that the detection by MIP@PS@CdTe probe was accurate and reliable. [Display omitted] •A novel fluorescence probe of MIP@PS@CdTe is fabricated for the detection of trace MG.•The fluorescence signal of CdTe QDs in PS-PDDA-QDs can be effectively amplified.•A core-shell MIP@PS@CdTe probe results in rapidity, high sensitivity and accuracy.