Gel permeation chromatography of sunflower pectin

Sunflower pectin has been fractionated on Sepharose 2B/Sepharose 4B. Molecular weights were measured within the eluate by light scattering and intrinsic viscosities to establish a universal calibration line, e.g. a plot of the logarithm of the product of the weight-average molecular weight and the i...

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Bibliographic Details
Published inCarbohydrate polymers Vol. 5; no. 4; pp. 241 - 250
Main Authors Anger, H., Berth, G.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 1985
Elsevier Science
Subjects
Biological and medical sciences
Food additives
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Fractionation
Sunflower seed
Raw materials
Pectin
Properties
Gel permeation chromatography
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Summary:Sunflower pectin has been fractionated on Sepharose 2B/Sepharose 4B. Molecular weights were measured within the eluate by light scattering and intrinsic viscosities to establish a universal calibration line, e.g. a plot of the logarithm of the product of the weight-average molecular weight and the intrinsic viscosity against the elution volume. It was found that the universal calibration line of pectin differs, if only modestly, from those of dextran and dextran sulphate. Intrinsic viscosities and molecular weights do not correlate in the region of high molecular weights concerning about 15% of the sample. In most instances with molecular weights below 100 000 a Mark-Houwink relation of [η] = 0·0851 M w 0·68 is valid.
ISSN:0144-8617
1879-1344
DOI:10.1016/0144-8617(85)90033-5