Short report: Antibody responses of mice immunized with a tetravalent dengue recombinant protein subunit vaccine

• Published in: The American journal of tropical medicine and hygiene Vol. 65; no. 2; pp. 159 - 161
• Main Authors: Simmons, M, Murphy, GS, Hayes, CG
• Format: Journal Article
• Language: English
• Published: Lawrence, KS ASTMH 01.08.2001; Allen Press
• Subjects: Animals; Antibodies, Viral - blood; Arboviroses; ATP-Binding Cassette Transporters; Bacterial Proteins - genetics; Biological and medical sciences; Carrier Proteins - genetics; Cross Reactions; Dengue - prevention & control; Dengue fevers; Dengue Virus - genetics; Dengue Virus - immunology; Escherichia coli; Escherichia coli Proteins; Human viral diseases; Immunization; Infectious diseases; Maltose-Binding Proteins; Medical sciences; Mice; Mice, Inbred BALB C; Monosaccharide Transport Proteins; Neutralization Tests; Recombinant Fusion Proteins - immunology; Tropical medicine; Tropical viral diseases; Vaccines, Combined; Vaccines, Subunit - immunology; Vaccines, Synthetic; Viral diseases; Viral Proteins - genetics; Viral Vaccines - immunology; Immune response; Antibody; Vaccination; Rodentia; Dengue virus 4; Dengue virus 3; Vaccine; Dengue virus 2; Dengue virus 1; Flavivirus; Virus; Immunoprophylaxis; Vertebrata; Experimental disease; Mammalia; Mouse; Immunological investigation; Dengue group virus; Animal; Recombinant protein; Flaviviridae
• ISSN: 0002-9637; 1476-1645
• DOI: 10.4269/ajtmh.2001.65.159

Recombinant proteins containing the B domain of dengue virus serotypes 1-4 fused to the maltose binding protein (MBP) of Escherichia coli were evaluated individually and as a tetravalent vaccine candidate in mice. Sera from mice immunized with monovalent DEN-MBP recombinant protein vaccines developed high titers of serotype homologous antibody in the enzyme-linked immunosorbent assay and the plaque-reduction neutralization test. Cross-reactive antibody titers were either several dilutions lower or not detectable. Sera from mice immunized with the tetravalent DEN subunit vaccine neutralized all 4 DEN viruses in the plaque-reduction neutralization test. The neutralizing antibody titers to each individual serotype were significantly greater than any cross-reactive neutralizing antibody titers induced by the monovalent vaccines, providing evidence that the tetravalent DEN recombinant subunit vaccine produced specific neutralizing antibody to all 4 serotypes of dengue virus.