The Kinesin-Related Protein Eg5 Associates with both Interphase and Spindle Microtubules during Xenopus Early Development

• Published in: Developmental biology Vol. 164; no. 1; pp. 147 - 159
• Main Authors: Houliston, Evelyn, Le Guellec, René, Kress, Michel, Philippe, Michel, Le Guellec, Katherine
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.1994; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Early stages. Segmentation. Gastrulation. Neurulation; Embryology: invertebrates and vertebrates. Teratology; Endoplasmic Reticulum - chemistry; Freshwater; Fundamental and applied biological sciences. Psychology; Interphase; Kinesin - analysis; Kinesin - genetics; Kinesin - physiology; Meiosis; Microtubule-Associated Proteins - analysis; Microtubule-Associated Proteins - physiology; Microtubules - physiology; Molecular Sequence Data; Oocytes - chemistry; Ovum - chemistry; Xenopus - embryology; Xenopus - metabolism; Xenopus laevis; Xenopus Proteins; Embryonic development; Interphase; Mitosis; Xenopus laevis; Amphibia; Cell division; Early stage; Salientia; Meiosis; Oogenesis; Vertebrata; Cytoskeleton; Microtubule associated protein; Microtubule; Localization; Kinesin; Quantitative analysis
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1006/dbio.1994.1187

We have examined the changing abundance and distribution of the kinesin-related protein Eg5 during oogenesis and early development in Xenopus laevis . Antibodies raised against proteins synthesized from parts of a novel Eg5 gene expressed in eggs were used for Western blotting and immunofluorescence. Eg5 protein was highly enriched in oocytes and eggs compared with other adult tissues. It accumulated during the latter stages of oogenesis and increased a further threefold during oocyte maturation. Its level then gradually declined during early development. In oocytes, eggs, and early embryos, Eg5 protein could be detected throughout the cytoplasm and in subcortical aggregates. Eg5 staining was found concentrated in meiotic and mitotic spindles, mainly toward the poles. Some Eg5 staining colocalized with microtubules in interphase cells, including the aligned subcortical microtubules in fertilized eggs implicated in the cortical rotation that specifies the dorsoventral axis. Interphase association of Eg5 with microtubules during early development was confirmed by copelleting the protein with microtubules from egg homogenates. In tadpoles and tissue culture cells, Eg5 colocalized with spindle microtubules throughout mitosis but not with interphase microtubules. These results suggest that the Eg5 microtubule motor may function in meiosis, mitosis, and interphase during early development.