Rejuvenation of Sequoia sempervirens in vitro: Changes in isoesterases and isoperoxidases

• Published in: Plant and cell physiology Vol. 37; no. 1; pp. 77 - 80
• Main Authors: Huang, H.J. (Academia Sinica, Taipei (Taiwan)), Chen, Y, Kuo, J.L, Kuo, T.T, Tzeng, C.C, Huang, B.L, Chen, C.M, Huang, L.C
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.01.1996
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; BOIS DE JEUNESSE; CULTIVO IN VITRO; CULTURE IN VITRO; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; Economic plant physiology; ESTERASAS; ESTERASE; Fundamental and applied biological sciences. Psychology; In vitro culture; ISOENZIMAS; ISOENZYME; Isoesterases; Isoperoxidases; MADERA JOVEN; PEROXIDASAS; PEROXYDASE; Phase reversal; Plant physiology and development; REGENERACION VEGETAL; Rejuvenation; RESTAURATION COUVERTURE VEGETALE; SEQUOIA SEMPERVIRENS; Shoot culture; Shoot-tip grafting; Tissue cultures, protoplasts; Enzyme; Isozyme; Rejuvenation; Rhizogenesis; Tissue culture; Biological marker; Esterases; In vitro; Sequoia sempervirens; Micrografting; Peroxidases; Restoration; Softwood forest tree; Gymnospermae; Hydrolases; Coniferales; Spermatophyta; Peroxidase; Developmental stage; Oxidoreductases
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a028916

Rejuvenation, as evidenced by restored rooting competence, of Sequoia sempervirens by a series of repeated grafting of adult shoot tips onto juvenile rootstocks in vitro was confirmed. Complete restoration of rooting competence was achieved after grafting repeatedly five times. The rejuvenation was correlated with a disappearance of adult-associated esterase and peroxidase isozymes and an appearance of isoesterases and isoperoxidases that were characteristic of juvenile-phase shoots. These esterase and peroxidase isozymes could serve as markers to assist phase change investigations