Antimicrobial and anticoagulation activity of silver nanoparticles synthesized from the culture supernatant of Pseudomonas aeruginosa

• Published in: Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 19; no. 4; pp. 1299 - 1303
• Main Authors: Jeyaraj, Muniyandi, Varadan, Senthilkumar, Anthony, Kevin John Pulikotil, Murugan, Muralidharan, Raja, Ashok, Gurunathan, Sangiliyandi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.07.2013; 한국공업화학회
• Subjects: Biochemistry; Culture; Dynamic light scattering; Escherichia coli; Nanoparticles; P. aeruginosa; Pseudomonas aeruginosa; Scanning electron microscopy; Scanning electron microscopy (SEM); Silver; Silver nanoparticles; Staphylococcus aureus; UV–vis spectroscopy; 화학공학; UV–vis spectroscopy; Dynamic light scattering; Scanning electron microscopy (SEM); Silver nanoparticles; P. aeruginosa
• ISSN: 1226-086X; 1876-794X
• DOI: 10.1016/j.jiec.2012.12.031

Here, we describe biosynthesis of silver nanoparticles by reduction of aqueous Ag+ ions with the culture supernatant of Pseudomonas aeruginosa. The morphological, physiological, biochemical and molecular level identification of the strain GS1 resembles P. aeruginosa. The nanoparticles synthesized by P. aeruginosa were characterized by UV–vis spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM). The size-distribution of nanoparticles was determined using a particle-size analyzer and the average particle-size was found to be 80nm. The biological activities of the synthesized silver nanoparticles like antimicrobial activity were confirmed against Escherichia coli and Staphylococcus aureus and it have stable anti-coagulant effect.