The design of strain-specific polymerase chain reactions for discrimination of the racoon rabies virus strain fron indigenous rabies viruses of Ontario

Since its recognition as a discrete epizootic in Florida in the early 1950s, the raccoon strain of rabies virus (RV) has spread over almost the entire eastern seaboard of the US and now threatens to enter the southernmost regions of Canada. To characterise this RV strain in more detail, nucleotide s...

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Bibliographic Details
Published inJournal of virological methods Vol. 57; no. 1; pp. 1 - 14
Main Authors Nadin-davis, Susan A., Huang, Wei, Wandeler, Alexander I.
Format Journal Article
LanguageEnglish
Published London Elsevier B.V 01.03.1996
Amsterdam Elsevier
New York, NY
Subjects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Polymerase chain reaction (PCR)
Rabies virus
Strain discrimination
Techniques used in virology
Virology
Polymerase chain reaction (PCR)
Strain discrimination
Rabies virus
Fissipedia
Carnivora
Typing
RNA
Nucleocapsid
Reverse transcription
Genotype
Glycoproteins
Identification
Method
Virus
Proteins
Polymerase chain reaction
Procyon lotor
Vertebrata
Mammalia
Rhabdoviridae
Lyssavirus
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Summary:Since its recognition as a discrete epizootic in Florida in the early 1950s, the raccoon strain of rabies virus (RV) has spread over almost the entire eastern seaboard of the US and now threatens to enter the southernmost regions of Canada. To characterise this RV strain in more detail, nucleotide sequencing of the N and G genes, encoding the nucleoprotein and glycoprotein, respectively, of representative isolates has been undertaken. This sequence information generated a conserved restriction map of the N gene, thereby permitting unequivocal identification of this strain by molecular techniques. Comparisons of the predicted nucleoprotein and glycoprotein products with those of other RV strains identified a number of amino acid sequence variations conserved only in the raccoon strain. This information was used to design strain-specific primers targeted to the N gene sequences encoding these residues. The incorporation of these primers into a multiplex polymerase chain reaction (PCR) protocol permitted easy and rapid discrimination between the raccoon RV strain and indigenous Ontario RVs.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(95)01907-3