Micro-scale temperature measurement method using fluorescence polarization

A novel method that can measure the fluid temperature in microscopic scale by measuring the fluorescence polarization is described in this paper. The measurement technique is not influenced by the quenching effects which appears in conventional LIF methods and is believed to show a higher reliabilit...

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Bibliographic Details
Published inJournal of physics. Conference series Vol. 745; no. 3; pp. 32097 - 32104
Main Authors Tatsumi, K., Hsu, C-H., Suzuki, A., Nakabe, K.
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.09.2016
Subjects
Fluorescence
Measurement methods
Measurement techniques
Microchannels
Physics
Polarization
Quenching
Temperature measurement
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Summary:A novel method that can measure the fluid temperature in microscopic scale by measuring the fluorescence polarization is described in this paper. The measurement technique is not influenced by the quenching effects which appears in conventional LIF methods and is believed to show a higher reliability in temperature measurements. Experiment was performed using a microchannel flow and fluorescent molecule probes, and the effects of the fluid temperature, fluid viscosity, measurement time, and pH of the solution on the measured fluorescence polarization degree are discussed to understand the basic characteristics of the present method. The results showed that fluorescence polarization is considerably less sensible to these quenching factors. A good correlation with the fluid temperature, on the other hand, was obtained and agreed well with the theoretical values confirming the feasibility of the method.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/745/3/032097