use of RAPD markers in Hordeum phylogeny

The phylogenetic relationships among 39 wild Hordeum species, subspecies, and cultivated barley were investigated using RAPD markers as discriminating characters. Seventy-six RAPD fragments were generated using 12 single decameric primers of arbitrary nucleotide sequences. Amplification reactions re...

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Bibliographic Details
Published inGenome Vol. 39; no. 4; pp. 646 - 654
Main Authors Marillia, E.F, Scoles, G.J
Format Journal Article
LanguageEnglish
Published Canada 01.08.1996
Subjects
genetic markers
genetic polymorphism
Hordeum
Hordeum vulgare
phylogeny
random amplified polymorphic DNA technique
species differences
wild plants
wild relatives
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Summary:The phylogenetic relationships among 39 wild Hordeum species, subspecies, and cultivated barley were investigated using RAPD markers as discriminating characters. Seventy-six RAPD fragments were generated using 12 single decameric primers of arbitrary nucleotide sequences. Amplification reactions resulted in fragments ranging in length between 200 and 2000 bp. Clearly resolved bands were scored for their presence or absence in a binary matrix. Amplified products were treated as independent characters to generate a phenogram using the NTSYS-PC package. Tree topology was generally found to be consistent with those based on morphological treatments. However, a few species like H. erectifolium, H. jubatum and, to a lesser extent, H. bulbosum occupied a position different from previous classifications. The results demonstrated that RAPD technology represents a useful and reliable tool for detecting polymorphism for phylogenetic studies.
ISSN:0831-2796
1480-3321
DOI:10.1139/g96-082