A new simple screening method for the detection of paralytic shellfish poisoning toxins

The current testing for paralytic shellfish poisoning (PSP) in shellfish is based on the mouse bioassay (MBA). To alleviate animal welfare concerns, we evaluated the utility of using sublethal indicators of toxicity as an alternative to measuring time to death. Live mice were injected with a PSP con...

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Bibliographic Details
Published inChinese journal of oceanology and limnology Vol. 30; no. 5; pp. 786 - 790
Main Author 程金平 皮帅帅 叶属峰 高昊旻 姚磊 蒋真毅 宋玉玲 席磊
Format Journal Article
LanguageEnglish
Published Heidelberg Springer-Verlag 01.09.2012
SP Science Press
Springer Nature B.V
Subjects
Acetylcholine
Animal welfare
Bio-assays
Bioassays
Biology
Blood
Blood tests
Congeners
death
Earth and Environmental Science
Earth Sciences
Food contamination & poisoning
Marine
mice
Neurotransmitters
Oceanography
Paralytic shellfish poisoning
Poisoning
PSP
screening
Shellfish
Toxicity
Toxins
乙酰胆碱
检查
检测
毒性指标
神经递质
麻痹性贝毒
麻痹性贝类毒素
paralytic shellfish poisoning (PSP)
acetylcholine
assay
mouse bioassay
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Summary:The current testing for paralytic shellfish poisoning (PSP) in shellfish is based on the mouse bioassay (MBA). To alleviate animal welfare concerns, we evaluated the utility of using sublethal indicators of toxicity as an alternative to measuring time to death. Live mice were injected with a PSP congener and the changes in neurotransmitter levels were measured 60, 90, and 120 min after injection. Acetylcholine (ACh) was the most sensitive marker for PSP toxicity. The changes in neurotransmitter levels were most pronounced in the blood. Thus, measurement of Ach levels in the blood may serve as a sensitive predictor for PSP that would not require sacrifice of the mice. This method was relatively simple, sensitive (less than 1 μg/kg weight, equivalent to 20 ng/mL), low maintenance, and rapid (less than 60 min).
Bibliography:37-1150/P
paralytic shellfish poisoning （PSP）; mouse bioassay; acetylcholine; assay
The current testing for paralytic shellfish poisoning （PSP） in shellfish is based on the mouse bioassay （MBA）. To alleviate animal welfare concerns, we evaluated the utility of using sublethal indicators of toxicity as an alternative to measuring time to death. Live mice were injected with a PSP congener and the changes in neurotransmitter levels were measured 60, 90, and 120 min after injection. Acetylcholine （ACh） was the most sensitive marker for PSP toxicity. The changes in neurotransmitter levels were most pronounced in the blood. Thus, measurement of Ach levels in the blood may serve as a sensitive predictor for PSP that would not require sacrifice of the mice. This method was relatively simple, sensitive （less than 1 ~g/kg weight, equivalent to 20 ng/mL）, low maintenance, and rapid （less than 60 min）.
http://dx.doi.org/10.1007/s00343-012-1097-8
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
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ISSN:0254-4059
2096-5508
1993-5005
2523-3521
DOI:10.1007/s00343-012-1097-8