association of human adipose angiotensinogen gene expression with abdominal fat distribution in obesity

• Published in: International Journal of Obesity Vol. 24; no. 6; pp. 673 - 678
• Main Authors: Harmelen, V. van, Elizalde, M, Ariapart, P, Bergstedt-Lindqvist, S, Reynisdottir, S, Hoffstedt, J, Lundkvist, I, Bringman, S, Arner, P
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.06.2000
• Subjects: Abdomen; abdominal fat; Adipocytes; Adipose tissue; Adipose Tissue - metabolism; Adipose Tissue - pathology; Adult; angiotensinogen; Angiotensinogen - genetics; Atherosclerosis; bariatric surgery; Biopsy; Body Composition; Body Constitution; Body fat; Body Mass Index; correlation; Female; Gastrointestinal surgery; Gastroplasty; Gene Expression; genes; Hospitals; Humans; Hypertension; Male; Medicin och hälsovetenskap; men; messenger RNA; metabolic diseases; Middle Aged; Obesity; Obesity - genetics; Obesity - surgery; Reverse Transcriptase Polymerase Chain Reaction; ribosomal RNA; RNA, Messenger - analysis; RNA, Ribosomal, 18S - analysis; waist-to-hip ratio; Weight control; weight loss; women; Sweden
• ISSN: 0307-0565; 1476-5497
• DOI: 10.1038/sj.ijo.0801217

OBJECTIVE: To investigate in obese subjects the relationship between angiotensinogen gene expression in the abdominal omental and subcutaneous adipose tissue on the one hand and body fat distribution as measured by waist-to-hip ratio (WHR) on the other hand and to compare angiotensinogen gene expression between the two adipose tissue regions. SUBJECTS: Twenty obese subjects undergoing weight reduction surgery with adjustable gastric banding (12 men, eight women; WHR 0.89-1.09; body mass index (BMI) 29-51 kg/m2, age 26-54 y). MEASUREMENTS: Omental and subcutaneous adipose angiotensinogen mRNA and 18S ribosomal RNA (reference gene) levels were measured by competitive quantitative reverse transcriptase-polymerase chain reaction. RESULTS: Angiotensinogen mRNA levels were one-third higher in the omental than in the subcutaneous adipose tissue region (P = 0.02). The 18S rRNA levels did not differ significantly between the two adipose tissue regions. WHR correlated positively and significantly with angiotensinogen mRNA in both the subcutaneous and the omental adipose tissue (r = 0.5). This relationship was independent of age and BMI. However, WHR did not correlate with 18S rRNA in any of the adipose tissue regions. CONCLUSION: The angiotensinogen gene in adipose tissue might be involved in the development of upper-body obesity.