Molecular cloning, characterization, expression and enzyme activity of catalase from planarian Dugesia japonica in response to environmental pollutants

• Published in: Ecotoxicology and environmental safety Vol. 165; pp. 88 - 95
• Main Authors: Zhang, He-Cai, Ma, Ke-Xue, Yang, Yu-Juan, Shi, Chang-Ying, Chen, Guang-Wen, Liu, De-Zeng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.12.2018
• Subjects: Amino Acid Sequence; Animals; Antioxidants - metabolism; Biomarker; Biomarkers - metabolism; Bromides - pharmacology; Catalase; Catalase - genetics; Catalase - metabolism; Cloning, Molecular; DNA, Complementary - metabolism; Dose-Response Relationship, Drug; Dugesia japonica; Environmental pollutants; Environmental Pollutants - pharmacology; Gene Expression - drug effects; Glycine - analogs & derivatives; Glycine - pharmacology; Glyphosate; Herbicides - pharmacology; Imidazoles - pharmacology; mRNA expression; Oxidation-Reduction; Oxidative Stress; Planarians - enzymology; RNA, Messenger - metabolism; Sequence Analysis, DNA; Up-Regulation - drug effects; Biomarker; Environmental pollutants; Dugesia japonica; Catalase; mRNA expression
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2018.08.083

Catalase (CAT) is an important antioxidant enzyme that protects aerobic organisms against oxidative damage by degrading hydrogen peroxide to oxygen and water. CAT mRNAs have been cloned from many species and employed as useful biomarkers of oxidative stress. In the present study, we cloned the cDNA sequence of CAT gene from freshwater planarian Dugesia japonica (designated as DjCAT) by means of RACE method. Sequence analysis and multiple alignment jointly showed that the full-length cDNA sequence consists of 1734 nucleotides, encoding 506 amino acids. Three catalytic amino acid residues of His71, Asn144 and Tyr354, two CAT family signature sequences of a proximal active site signature (60FDRERIPERVVHAKGGGA77) and a heme-ligand signature motif (350RLFSYRDTQ358) are highly conserved, suggesting that the DjCAT belongs to the NADPH and heme-binding CAT family and has similar functions. In addition, the transcriptional level of CAT gene and activity of CAT enzyme upon acute exposure of environmental pollutants glyphosate and 1-decyl-3-methylimidazolium bromide ([C10mim]Br) were investigated systematically. The variation of CAT mRNA expression in D. japonica was quantified by real-time PCR and the results indicated that it was up-regulated after exposure to glyphosate or [C10mim]Br with a dose-dependent manner but not linearly. Even though the variation trend of CAT activity upon glyphosate stress was not monotonously increased and inconsistent with that after [C10mim]Br exposure on day 1 and 3 sampling time, with the duration prolonged to day 5 they both presented a dose-dependent increase and the differences achieved extreme significance in all treated groups compared to the control. These findings suggested that DjCAT plays an important role in antioxidant defense in D. japonica, and the mRNA expression of CAT would also be used as an effective biomarker to monitor the pollution in aquatic environment just like its corresponding enzyme. •The cDNA of CAT was firstly cloned and characterized from planarian Dugesia japonica.•mRNA expression of CAT and enzyme activity of CAT in response to pollutants were explored.•CAT plays an important role in antioxidant defense in Dugesia japonica.•CAT mRNA expression would be used as an effective biomarker in pollution monitoring.