Nicotinic acid- N-glucoside in heterotrophic parsley cell suspension cultures

• Published in: Phytochemistry (Oxford) Vol. 27; no. 11; pp. 3489 - 3493
• Main Authors: Upmeier, Barbara, Thomzik, Jürgen E., Barz, Wolfgang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 1988; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carbohydrates; Fundamental and applied biological sciences. Psychology; nicotinic acid; nicotinic acid- N-glucoside; Other biological molecules; Petroselinum hortense; plant cell cultures; Umbelliferae; plant cell cultures; Umbelliferae; Petroselinum hortense; nicotinic acid; nicotinic acid- N-glucoside; Cell culture; Suspension culture; Vegetals; Dicotyledones; Angiospermae; Spermatophyta; Biosynthesis; Glycoside; Heteroside
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/0031-9422(88)80754-4

Heterotrophic cell suspension cultures of Petroselinum hortense rapidly convert exogenously applied nicotinic acid to nicotinic acid- N-β- d-glucopyranoside and not to the previously reported nicotinic acid- N-arabinoside. The structure of the N-glucoside was determined by spectroscopic techniques and comparison with synthetic material. The nicotinic acid- N-glucoside was also found to be an endogenous constituent of parsley cells at a level of ca 35–40 nmol/9 fr. wt. Application of [U- 14C]-aspartate to parsley cell cultures led to the accumulation of labelled nicotinic acid- N-glucoside, which was subsequently turned over with a half life of 24 hr indicating rapid metabolism of the N-glucoside.