Complex Expression Pattern of the TNF Region Gene LST1 through Differential Regulation, Initiation, and Alternative Splicing

• Published in: Genomics (San Diego, Calif.) Vol. 45; no. 3; pp. 591 - 600
• Main Authors: de Baey, Annegret, Fellerhoff, Barbara, Maier, Sabine, Martinozzi, Silvia, Weidle, Ullrich, Weiss, Elisabeth H.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.11.1997; Elsevier
• Subjects: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Blood Proteins - genetics; Blood Proteins - metabolism; Cell Line; Chromosome Mapping; Cloning, Molecular; DNA, Complementary; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Genes. Genome; Humans; Macrophages - physiology; Membrane Proteins; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Polymerase Chain Reaction - methods; Transcription, Genetic; Tumor Necrosis Factor-alpha - genetics; Alternative splicing; Vertebrata; Regulation(control); Mammalia; Monocyte; Mouse; Nucleotide sequence; Leukocyte; Rodentia; Homology; Gene organization; Gene expression
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1997.4963

Recently, a novel gene, LST1, was identified in the tumor necrosis factor region of the HLA complex, 4 kb centromeric of the lymphotoxin β gene. By analyzing several full-length cDNA clones and the genomic DNA, we identified seven exons and four introns, spanning 2.7 kb. Isolation of mouse LST1 cDNA clones established the open reading frame. LST1 transcription is characterized by four alternative transcription initiation sites and extensive alternative splicing. The derived polypeptides vary with regard to the presence of the hydrophobic N-terminus and in short internal sequences. In addition, alternative splicing results in LST1 mRNAs encoding different carboxy-terminal sequences. LST1 is predominantly transcribed in monocytes, and mRNA levels increase upon stimulation with interferon-γ, with a concomitant change in the mRNA pattern resulting in an enhanced expression of the short LST1 transcripts. These data suggest that LST1 may have a specific role in monocytes and possibly also in T cells. Moreover, we found that the recently published cDNA 1C7 is encoded just centromeric of LST1.