In vitro degradation and protein release of semi-IPN hydrogels consisted of poly(acrylic acid-acrylamide-methacrylate) and amylose

• Published in: Journal of applied polymer science Vol. 105; no. 6; pp. 3432 - 3438
• Main Authors: Li, Shengfang, Yang, Yajiang, Yang, Xiangliang, Xu, Huibi
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.09.2007; Wiley
• Subjects: Applied sciences; Biological and medical sciences; Exact sciences and technology; General pharmacology; in vitro degradation; Medical sciences; Natural polymers; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Physicochemistry of polymers; protein release; semi-IPN hydrogel; Starch and polysaccharides; Biological properties; Acrylic acid copolymer; Biodegradability; Control release polymer; Drug carrier; Serum albumin; Semiinterpenetrating polymer network; Experimental study; Methyl methacrylate copolymer; Protein; Colloidal gel; Polyelectrolyte; Amylose; protein release; semi-IPN hydrogel; Enzymatic digestion; Ethyl methacrylate copolymer; Oside polymer; Hydrogel; in vitro degradation; Release; Butyl methacrylate copolymer
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.26389

The enzymatic degradation mechanism of semi‐interpenetrating network (semi‐IPN) hydrogel of poly (acrylic acid‐acrylamide‐methacrylate) crosslinked by azocompound and amylose in vitro was investigated in the presence of Fungamyl 800L (α‐amylase) and rat cecum content (cecum bacteria). The degradation mechanism involves degradable competition, i.e., reduction of azo crosslinkage is dominant in the earlier period of degradation. Subsequently, the degradation of gels is continued by combination of reduction of azo crosslinkage and hydrolysis of amylose. The cumulative release ratios of Bovine serum albumin (BSA, as a model drug) loaded semi‐IPN gels are 25% in pH 2.2 buffer solutions and 74% in pH 7.4 buffer solutions within 48 h. Moreover, the release behavior of BSA from the semi‐IPN gels indicates that it follows Fickian diffusion mechanism in pH 2.2 media and non‐Fickian diffusion and polymer chains relaxation mechanism in pH 7.4 media. The results indicate that the release of BSA from the semi‐IPN gels was controlled via a combined mechanism of pH dependent swelling and specificity to enzymatic degradation. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007