Discovery proteomics of human placental tissue

We describe a label‐free proteomics protocol for the interrogation of the placental proteome. Step‐by‐step directions, including tissue cleanup and preparation, proteolytic digestion, nanoLC–MS/MS data collection and data analysis, are provided. The workflow has been applied toward exploring differe...

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Bibliographic Details
Published inRapid communications in mass spectrometry Vol. 38; no. S1; pp. e9189 - n/a
Main Authors Mellinger, Allyson L., McCoy, Krista, Minior, Duy An T., Williams, Taufika Islam
Format Journal Article
LanguageEnglish
Published England Wiley Subscription Services, Inc 01.02.2023
Subjects
Data analysis
Data collection
Female
Humans
Interrogation
Placenta - metabolism
Pregnancy
Proteolysis
Proteome - metabolism
Proteomics
Proteomics - methods
Tandem Mass Spectrometry
Workflow
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Summary:We describe a label‐free proteomics protocol for the interrogation of the placental proteome. Step‐by‐step directions, including tissue cleanup and preparation, proteolytic digestion, nanoLC–MS/MS data collection and data analysis, are provided. The workflow has been applied toward exploring differential protein expression patterns in placentas from women who have been exposed to drugs during pregnancy relative to those who have not. We collected 20 tissue specimens, each representing a combination of spatially diverse sections across the placenta. These specimens were analyzed in the work described here, to survey information across the entire organ. This protocol can be scaled up or down as needed.
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Current address: Harbor Branch Oceanographic Institute, Florida Atlantic University, 5600 US 1 North, Ft. Pierce, Florida 34946
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.9189