Electron microscopic studies on liver 5‐aminolaevulinate synthase

The structure of chick embryo liver 5‐aminolaevulinate synthase has been examined by electron microscopic studies using negative staining. From the different projections of the enzyme particles observed in election micrographs, a model for the enzyme molecule has been proposed. In this model, an enz...

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Bibliographic Details
Published inEuropean journal of biochemistry Vol. 144; no. 3; pp. 577 - 579
Main Authors PIROLA, Byron A., MAYER, Frank, BORTHWICK, Iain A., SRIVASTAVA, Gopesh, MAY, Brian K., ELLIOTT, William H.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 02.11.1984
Blackwell
Subjects
5-Aminolevulinate Synthetase - analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chick Embryo
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Liver - enzymology
Liver - ultrastructure
Lyases
Microscopy, Electron
Models, Structural
Lyase
Embryo
Vertebrata
Molecular structure
Enzyme
Animal
Liver
Δ-Aminolaevulinate synthase
Models
Chicken
Aves
Electron microscopy
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Summary:The structure of chick embryo liver 5‐aminolaevulinate synthase has been examined by electron microscopic studies using negative staining. From the different projections of the enzyme particles observed in election micrographs, a model for the enzyme molecule has been proposed. In this model, an enzyme molecule consists of two curved and identical subunits associated in opposite polarities. From the dimensions of an enzyme molecule subunit measured from electron micrographs, the relative molecular mass of each subunit is estimated to be 70000.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2956
1432-1033
DOI:10.1111/j.1432-1033.1984.tb08504.x