SRSF1 promotes vascular smooth muscle cell proliferation through a Δ133p53/EGR1/KLF5 pathway

• Published in: Nature communications Vol. 8; no. 1; p. 16016
• Main Authors: Xie, Ning, Chen, Min, Dai, Rilei, Zhang, Yan, Zhao, Hanqing, Song, Zhiming, Zhang, Lufeng, Li, Zhenyan, Feng, Yuanqing, Gao, Hua, Wang, Li, Zhang, Ting, Xiao, Rui-Ping, Wu, Jianxin, Cao, Chun-Mei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.08.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 13/95; 45; 45/91; 631/443/592; 631/80/86; 82; 96; 96/2; Animals; Aorta - cytology; Carotid Artery Injuries - pathology; Carotid Artery, Common - pathology; Cell Cycle; Cell Proliferation - genetics; Cells, Cultured; Coronary Vessels - cytology; Early Growth Response Protein 1 - metabolism; Gene Knockdown Techniques; Humanities and Social Sciences; Humans; Kruppel-Like Transcription Factors - metabolism; Mammary Arteries; Mice; Mice, Knockout; multidisciplinary; Muscle, Smooth, Vascular - cytology; Myocytes, Smooth Muscle - cytology; Neointima - genetics; Protein Isoforms; Rats; Science; Science (multidisciplinary); Serine-Arginine Splicing Factors - genetics; Signal Transduction; Tumor Suppressor Protein p53 - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms16016

Though vascular smooth muscle cell (VSMC) proliferation underlies all cardiovascular hyperplastic disorders, our understanding of the molecular mechanisms responsible for this cellular process is still incomplete. Here we report that SRSF1 (serine/arginine-rich splicing factor 1), an essential splicing factor, promotes VSMC proliferation and injury-induced neointima formation. Vascular injury in vivo and proliferative stimuli in vitro stimulate SRSF1 expression. Mice lacking SRSF1 specifically in SMCs develop less intimal thickening after wire injury. Expression of SRSF1 in rat arteries enhances neointima formation. SRSF1 overexpression increases, while SRSF1 knockdown suppresses the proliferation and migration of cultured human aortic and coronary arterial SMCs. Mechanistically, SRSF1 favours the induction of a truncated p53 isoform, Δ133p53, which has an equal proliferative effect and in turn transcriptionally activates Krüppel-like factor 5 (KLF5) via the Δ133p53-EGR1 complex, resulting in an accelerated cell-cycle progression and increased VSMC proliferation. Our study provides a potential therapeutic target for vascular hyperplastic disease. The hyperproliferation of vascular smooth muscle cells underlies many vascular diseases. Here Xie et al . show that the splicing factor SRSF1 is an endogenous stimulator of human and mouse aortic smooth muscle cell proliferation via the Δ133p53/EGR1/KLF5 signalling axis, identifying potential therapeutic targets for vascular proliferative disorders.