Human cardiac and skeletal muscle spectrins: differential expression and localization

We describe multiple human cardiac and skeletal muscle spectrin isoforms. Cardiac muscle expresses five erythroid alpha,beta spectrin-reactive isoforms with estimated MR's of 280, 274, 270, 255, and 246 kD, respectively. At least one nonerythroid alpha-spectrin of MR 284 kD is expressed in hear...

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Published inCell motility and the cytoskeleton Vol. 21; no. 4; p. 293
Main Authors Vybiral, T, Winkelmann, J C, Roberts, R, Joe, E, Casey, D L, Williams, J K, Epstein, H F
Format Journal Article
LanguageEnglish
Published United States 1992
Subjects
Adult
Antibodies
Electrophoresis, Polyacrylamide Gel
Humans
Immunohistochemistry
Muscles - chemistry
Myocardium - chemistry
Neuromuscular Junction - chemistry
Spectrin - analysis
Spectrin - isolation & purification
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Summary:We describe multiple human cardiac and skeletal muscle spectrin isoforms. Cardiac muscle expresses five erythroid alpha,beta spectrin-reactive isoforms with estimated MR's of 280, 274, 270, 255, and 246 kD, respectively. At least one nonerythroid alpha-spectrin of MR 284 kD is expressed in heart. While skeletal muscle shares the 280, 270, and 246 kD erythroid spectrins, it expresses an immunologically distinct 284 kD nonerythroid alpha-spectrin isoform. The 255 kD erythroid beta-spectrin isoform is specific for cardiac tissue. By immunocytochemistry, both erythroid beta- and nonerythroid alpha-spectrins are localized to costameres, the plasma membrane, and the neuromuscular junctional region.
ISSN:0886-1544
DOI:10.1002/cm.970210405