Protocol for extracellular recordings in the external globus pallidus of the behaving/awake monkey

• Published in: STAR protocols Vol. 5; no. 2; p. 103081
• Main Authors: Katabi, Shiran, Israel, Zvi, Bergman, Hagai, Deffains, Marc
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 21.06.2024; Elsevier
• Subjects: behavior; cognitive neuroscience; Life Sciences; neuroscience; Protocol; cognitive neuroscience; behavior; neuroscience
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2024.103081

Extracellular recordings in behaving animals are useful for establishing associations between neuronal activity and behavior. Here, we describe how to record in the external globus pallidus (GPe) of monkeys engaged in a behavioral task. We detail the stereotaxic surgery for chamber and head-holder implantation, the post-operative MRI scan to ascertain the GPe coordinates and validate the position of the chamber, and the data collection. This protocol makes it possible to examine the electrophysiological features of GPe neurons in behaving monkeys. For complete details on the use and execution of this protocol, please refer to Katabi et al.1 [Display omitted] •Procedures for manufacturing microelectrodes and localizing the NHP GPe•Description of stereotaxic surgery for chamber and head-holder implantation•Steps for performing post-operative anatomical MRI scan•Instructions for recording extracellular neuronal activity in the NHP GPe Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Extracellular recordings in behaving animals are useful for establishing associations between neuronal activity and behavior. Here, we describe how to record in the external globus pallidus (GPe) of monkeys engaged in a behavioral task. We detail the stereotaxic surgery for chamber and head-holder implantation, the post-operative MRI scan to ascertain the GPe coordinates and validate the position of the chamber, and the data collection. This protocol makes it possible to examine the electrophysiological features of GPe neurons in behaving monkeys.