Genotypic variation in cell wall composition in a diverse set of 244 accessions of Miscanthus

• Published in: Biomass & bioenergy Vol. 35; no. 11; pp. 4740 - 4747
• Main Authors: Allison, Gordon G., Morris, Catherine, Clifton-Brown, John, Lister, Susan J., Donnison, Iain S.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2011; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Applied sciences; Biological and medical sciences; Biomass; breeding; cell wall components; Cellulose; Energy; Energy grass; Exact sciences and technology; Feedstock matching; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetics and breeding of economic plants; genotype; Hemicellulose; Lignin; Miscanthus; Natural energy; new variety; Plant cell wall; Plant material; Wales; United Kingdom; Great Britain; Europe; Western Europe; Feedstock matching; Lignin; Hemicellulose; Plant cell wall; Energy grass; Cellulose; Monocotyledones; Genetic variability; Interspecific comparison; Biomass; Cell wall; Energy crop; Gramineae; Angiospermae; Spermatophyta
• ISSN: 0961-9534; 1873-2909
• DOI: 10.1016/j.biombioe.2011.10.008

We report on a replicated spaced-plant trial in Wales of 244 diverse Miscanthus accessions grouped by classical taxonomy into M. sinensis, M. sacchariflorus and M. x giganteus. Large differences in cell wall composition were detected between taxonomic groups. Significantly higher concentrations of NDF (88.19, 85.59 and 85.03 %DW; S.E.D. of 0.300; P < 0.001); ADL (11.76, 10.15 and 9.07 %DW; S.E.D. of 0.087; P < 0.001) and cellulose (47.09, 42.94 and 42.50 %DW; S.E.D. of 0.255; P < 0.001); and significantly lower concentrations of hemicellulose (29.43, 32.50 and 33.46 %DW; S.E.D. of 0.130; P < 0.001) were present in the samples from M. x giganteus compared to the other groups. Growth year and the interaction between taxonomic group and growth year had smaller significant effects. Strong correlations were found between ADL and cellulose ( R = 0.72), and hemicellulose ( R = −0.76) in the M. x giganteus samples but correlations were weaker for M. sacchariflorus ( R = 0.66 and −0.59) and M. sinensis ( R = 0.46 and −0.40). Eleven genotypes had been assessed previously in the U.K. during the European Miscanthus Improvement (EMI) programme. These generally had higher concentrations of ADL and hemicellulose, and lower concentrations of cellulose than were reported for the U.K. site during the EMI study. These results suggest that cell wall composition and ADL concentration in particular, is highly stable between growth years but is affected by site location. Furthermore, there are differences in cell wall composition between Miscanthus taxonomic groups that may prove valuable for breeding new varieties that are compositionally matched to conversion process. ► We report on the cell wall composition of 244 mature Miscanthus genotypes grown in the UK over 3 consecutive growth years. ► We detected large significant differences in the concentrations of lignin, cellulose and hemicellulose between species. ► We detected lesser but significant differences in the concentration of cell wall components between growth years. ► These results inform on diversity within this largely undomesticated crop and have relevance for breeding improvement.