Structural features and antitumor activity of a novel polysaccharide from alkaline extract of Phellinus linteus mycelia

• Published in: Carbohydrate polymers Vol. 115; pp. 472 - 477
• Main Authors: Pei, Juan-Juan, Wang, Zhen-Bin, Ma, Hai-Le, Yan, Jing-Kun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 22.01.2015
• Subjects: Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antitumor activity; Cell Line, Tumor; Humans; Hydrogen-Ion Concentration; Molecular Weight; Phellinus linteus; Plant Extracts; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - pharmacology; Purification; Structure; Structure-Activity Relationship; Phellinus linteus; Antitumor activity; Purification; Structure; Polysaccharide
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.09.017

•PL-N1, a novel polysaccharide, purified from alkaline extract of P. linteus mycelia.•The fine structure of PL-N1 was elucidated by chemical and instrumental analyses.•PL-N1 possesses potential antitumor activity against the growth of HepG2 in vitro.•PL-N1 could be developed as a potential, natural antitumor agent and functional food A novel high molecular weight polysaccharide (PL-N1) was isolated from alkaline extract of the cultured Phellinus linteus mycelia. The weight average molecular weight (Mw) of PL-N1 was estimated at 343,000kDa. PL-N1 comprised arabinose, xylose, glucose, and galactose in the molar ratio of 4.0:6.7:1.3:1.0. The chemical structure of PL-N1 was investigated by FTIR and NMR spectroscopies and methylation analysis. The results showed that the backbone of PL-N1 comprised (1→4)-linked β-D-xylopyranosyl residues, (1→2)-linked α-D-xylopyranosyl residues, (1→4)-linked α-D-glucopyranosyl residues, (1→5)-linked β-D-arabinofuranosyl residues, (1→4)-linked β-D-xylopyranosyl residues which branched at O-2, and (1→4)-linked β-D-galactopyranosyl residues which branched at O-6. The branches consisted of (1→)-linked α-D-arabinofuranosyl residues. Antitumor activity assay in vitro showed that PL-N1 could inhibit the growth of HepG2 cells to a certain extent in a dose-dependent manner. Thus, PL-N1 may be developed as a potential, natural antitumor agent and functional food.