A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry

• Published in: Talanta (Oxford) Vol. 154; pp. 511 - 519
• Main Authors: Rocha, Bruno Alves, da Costa, Bruno Ruiz Brandão, de Albuquerque, Nayara Cristina Perez, de Oliveira, Anderson Rodrigo Moraes, Souza, Juliana Maria Oliveira, Al-Tameemi, Maha, Campiglia, Andres Dobal, Barbosa Jr, Fernando
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2016
• Subjects: Benzhydryl Compounds; Biomonitoring; Bisphenol A; Bisphenols; Brazil; Chromatography, Liquid; Dispersion; Dispersive liquid-liquid microextraction; Fast method; Human; Humans; Liquid chromatography; Liquid chromatography-tandem mass spectrometry; Liquid Phase Microextraction; Liquid-liquid extraction; Mass spectrometry; Phenols; Tandem Mass Spectrometry; Urine; Urine; Bisphenol A; Dispersive liquid-liquid microextraction; Liquid chromatography-tandem mass spectrometry; Biomonitoring; Fast method
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2016.03.098

In this study, a novel method combining dispersive liquid-liquid microextraction (DLLME) and fast liquid chromatography coupled to mass spectrometry (LC-MS/MS) was developed and validated for the extraction and determination of bisphenol A (BPA) and six bisphenol analogues, namely bisphenol S (BPS), bisphenol F (BPF), bisphenol P (BPP), bisphenol Z (BPZ), bisphenol AP (BPAP) and bisphenol AF (BPAF) in human urine samples. Type and volume of extraction and disperser solvents, pH sample, ionic strength, and agitation were evaluated. The matrix-matched calibration curves of all analytes were linear with correlation coefficients higher than 0.99 in the range level of 0.5–20.0ngmL−1. The relative standard deviation (RSD), precision, at three concentrations (1.0, 8.0 and 15.0ngmL−1) was lower than 15% with accuracy ranging from 90 to 112%. The biomonitoring capability of the new method was confirmed with the analysis of 50 human urine samples randomly collected from Brazilians. BPA was detected in 92% of the analyzed samples at concentrations ranging <LOQ to 10.4ngmL−1. The detection rates of BPS (10%), BPAF (4%) and BPF (2%) were much lower than 92%. [Display omitted] •A fast method for bisphenol A and six analogues determination in urine is proposed.•Method is based on DLLME sample preparation and LC-MS/MS.•The proposed method was applied for the analysis of urine samples of Brazilians.