Vitronectin-induced haptotaxis of vascular smooth muscle cells in Vitro

• Published in: Experimental cell research Vol. 194; no. 1; pp. 154 - 156
• Main Authors: Naito, Michitaka, Hayashi, Toshio, Funaki, Chiaki, Kuzuya, Masafumi, Asai, Kanichi, Yamada, Kazuyoshi, Kuzuya, Fumio
• Format: Journal Article
• Language: English
• Published: Orlando, FL Elsevier Inc 01.05.1991; Elsevier
• Subjects: Animals; Biological and medical sciences; Cattle; Cell Movement - drug effects; Cell physiology; Cells, Cultured; chemotaxis; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Glycoproteins - pharmacology; Molecular and cellular biology; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - drug effects; smooth muscle; Vitronectin; Cell culture; Smooth muscle; Stimulation; Glycoproteins; Biological transport
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(91)90145-K

Vitronectin, a multifunctional glycoprotein present in the plasma and interstitial tissues, has recently been found to be localized in atherosclerotic lesions. In this study we examined the effects of vitronectin on the migration of cultured bovine aortic smooth muscle cells using a modified Boyden chamber assay. The cells migrated to fluid-phase vitronectin in a concentration-dependent fashion. The cells also migrated to membrane filter surfaces precoated with vitronectin for a few minutes in the absence of additional vitronectin in the fluid phase, suggesting that this substance binds easily to the filters and stimulates cell migration by haptotaxis under the conditions described. These observations suggest that vitronectin deposited in the intima may be involved in the pathogenesis of atherosclerosis by recruiting smooth muscle cells from the media into the intima.