Matrix metalloproteinases in human gliomas: activation of matrix metalloproteinase-2 (MMP-2) may be correlated with membrane-type-1 matrix metalloproteinase (MT1-MMP) expression

• Published in: Journal of Korean medical science Vol. 15; no. 3; pp. 309 - 314
• Main Authors: Hur, J H, Park, M J, Park, I C, Yi, D H, Rhee, C H, Hong, S I, Lee, S H
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Academy of Medical Sciences 01.06.2000
• Subjects: Animals; Blotting, Northern - methods; Brain - pathology; Brain Neoplasms - enzymology; Brain Neoplasms - pathology; Enzyme Activation; Gene Expression Regulation, Enzymologic; Glioma - enzymology; Glioma - pathology; Humans; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; Matrix Metalloproteinases, Membrane-Associated; Metalloendopeptidases - genetics; Papio; Tissue Inhibitor of Metalloproteinase-1 - genetics; Tissue Inhibitor of Metalloproteinase-2 - genetics; Tumor Cells, Cultured
• ISSN: 1011-8934; 1598-6357
• DOI: 10.3346/jkms.2000.15.3.309

To evaluate possible roles of matrix metalloproteinase (MMP)-1, -2, tissue inhibitor of metalloproteinase (TIMP)-1, -2 and membrane-type-1 matrix metalloproteinase (MT1-MMP) in invasion of human gliomas, expressions of these proteins were investigated in ten cases of human glioma and two meningioma tissues and eight human glioma cell lines. In gelatin zymography, MMP-2 activities of glioblastomas were higher than astrocytomas. The activated form of MMP-2 was seen in five of six cases of glioblastomas, but not in astrocytomas. MMP-9 activity was detected in all cases of malignant astrocytomas but the reactivity of MMP-9 was weaker than that of MMP-2. MT1-MMP mRNA expression in glioblastomas was higher than that in astrocytomas. Five cases of glioblastomas with activated form of MMP-2 had MT1-MMP expressions. In vitro, human glioma cell lines with high expression of MT1-MMP also showed high MMP-2 activity. TIMP-1 transcripts were constitutively present in almost all glioma tissues and cell lines, whereas TIMP-2 mRNA were weak especially in malignant gliomas. Imbalance of TIMP-2/MMP-2 was observed using immunoprecipitation analysis in a glioma cell line. High expression of MMP-2 and MT1-MMP is possibly involved in invasiveness of malignant glioma.