Effects of Dietary Zinc Chloride and Zinc Sulfate on Life History Performance and Hemolymph Metabolism of Spodoptera litura (Lepidoptera: Noctuidae)

• Published in: Insects (Basel, Switzerland) Vol. 15; no. 9; p. 687
• Main Authors: Qi, Jingwei, Xia, Zhenzhou, Yang, Yang, Li, Chuanren, Wang, Zailing
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.09.2024; MDPI
• Subjects: Adults; Agricultural pests; Agriculture; Animal husbandry; Ascorbic acid; Biological activity; Biological effects; Chitin; Comparative analysis; Crop damage; Developmental stages; Diet; Environmental aspects; Fecundity; Females; Fertilizers; heavy metal stress; Heavy metals; Hemolymph; Insects; Larvae; Life history; Life tables; Low concentrations; Metabolism; Metabolites; Metabolomics; Nutrition research; Physiological aspects; Physiology; Signal transduction; Soybeans; Spodoptera litura; Sulfates; Survival; Toxicity; two-sex life table; untargeted metabolomics; Vitamins; Zinc; Zinc chloride; Zinc compounds; Zinc sulfate; China; Japan; heavy metal stress; untargeted metabolomics; Spodoptera litura; two-sex life table
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects15090687

Zinc is an essential micronutrient crucial in various biological processes of an organism. However, the effects of zinc vary depending on its chemical form. Therefore, the aim of this study was to conduct a comparative analysis of the life history performances and hemolymph metabolism of exposed to different concentrations of dietary zinc chloride (ZnCl ) and zinc sulfate (ZnSO ), utilizing two-sex life tables and untargeted metabolomics. The preadult survival rate of significantly decreased, while the preadult developmental period of was prolonged as the dietary ZnCl concentration increased. However, the fecundity of at 50 mg/kg dietary ZnCl was significantly increased. The intrinsic rate of increase ( ) and the finite rate of increase (λ) in in the control group (CK, no exogenous ZnCl or ZnSO added) and with 50 mg/kg dietary ZnCl were significantly higher than those at 100 mg/kg, 200 mg/kg, and 300 mg/kg. Dietary ZnSO exerts a devastating effect on the survival of . Even at the lowest concentration of 50 mg/kg dietary ZnSO , only 1% of could complete the entire life cycle. Furthermore, as the dietary ZnSO concentration increased, the developmental stage achievable by the larvae declined. High-throughput untargeted metabolomics demonstrated that both 100 mg/kg dietary ZnCl and ZnSO decreased the hemolymph vitamins levels and increased the vitamin C content, thereby helping larvae to counteract the stress induced by ZnCl and ZnSO . Simultaneously, dietary ZnCl obstructed the chitin synthesis pathway in the hemolymph of , thus extending the developmental period of larvae. These results indicate that low concentrations of Zn positively impact populations of , but the effectiveness and toxicity of Zn depend on its chemical form and concentration.