Antimicrobial Susceptibility and Molecular Typing of Multiple Chlamydiaceae Species Isolated from Genital Infection of Women in Egypt

• Published in: Microbial drug resistance (Larchmont, N.Y.) Vol. 18; no. 4; pp. 44 - 445
• Main Authors: Osman, Kamelia M., Ali, Hadia A., Eljakee, Jakeen A., Gaafar, Maha M., Galal, Hussein M.
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.08.2012
• Subjects: Adult; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Antimicrobial agents; Bacteria; Bacterial Typing Techniques; Cercopithecus aethiops; Chick Embryo; Chlamydia; Chlamydia Infections - drug therapy; Chlamydia Infections - epidemiology; Chlamydia Infections - microbiology; Chlamydia trachomatis; Chlamydiaceae; Chlamydiaceae - classification; Chlamydiaceae - drug effects; Chlamydiaceae - genetics; Chlamydiaceae - isolation & purification; Chlamydophila abortus; Chlamydophila psittaci; Cotton; Deoxyribonucleic acid; Disease; DNA; Drug resistance; Egypt - epidemiology; Embryos; Erythromycin; Erythromycin - pharmacology; Female; Humans; Infection; Microbial Sensitivity Tests; Monoclonal antibodies; outer membrane proteins; Penicillin; Penicillins - pharmacology; Polymerase Chain Reaction; Prevalence; Sexually transmitted diseases; STD; Tetracycline - pharmacology; Tetracyclines; Tissue culture; Typing; Vagina; Vagina - drug effects; Vagina - microbiology; Vero Cells; Women; Egypt
• ISSN: 1076-6294; 1931-8448
• DOI: 10.1089/mdr.2011.0235

This study investigated the existence of vaginal Chlamydia infection and the prevalence of the disease in symptomatic gynecologically diseased women in Egypt. In addition, the antibiotics penicillin, tetracycline, and erythromycin were evaluated for their in vitro antichlamydial activity of the isolated strains. Vaginal swabs ( n =160) were collected from females gynecologically diseased using cotton swabs. Samples were tested for Chlamydia by Vero cells tissue culture, chicken embryo, Gimenez staining, direct fluorescein-conjugated monoclonal antibody staining, and immunoperoxidase. Polymerase chain reaction (PCR) analyses conducted for the presence of chlamydial DNA was used to detect its specific DNA by the omp2 gene. PCR analyses conducted for the presence of chlamydial DNA revealed that 112/160 (70%) were positive for Chlamydiaceae . The specific DNA defined by the omp2 gene identified them as Chlamydia trachomatis (17/112, 15.2%), Chlamydophila psittaci (56/112, 50.0%), and Chlamydophila abortus (40/112, 35.7%). The antibiotics penicillin, tetracycline, and erythromycin at different concentrations were effective in inactivating the viability of Chlamydiaceae isolates.