Single nucleotide polymorphisms in intron 1 and intron 2 of Larimichthys crocea growth hormone gene are correlated with growth traits

• Published in: Chinese journal of oceanology and limnology Vol. 30; no. 2; pp. 279 - 285
• Main Author: 倪静 尤锋 许建和 徐冬冬 文爱韵 吴志昊 徐永立 张培军
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.03.2012; SP Science Press; Springer Nature B.V
• Subjects: Animal growth; Animal morphology; Biology; Body height; Conformation; Correlation; Earth and Environmental Science; Earth Sciences; Fish; Gene polymorphism; genes; Genetic diversity; Genetic markers; Genotypes; Growth hormone; Growth hormones; growth traits; Haplotypes; Height; Hormones; introns; Larimichthys crocea; Marine; Marker-assisted selection; Nucleotide sequence; Nucleotides; Oceanography; Physical growth; Polymorphism; Single-nucleotide polymorphism; somatotropin; Stocks; 内含子; 单核苷酸多态性; 单链构象多态性; 大黄鱼; 标记辅助选择; 生长性状; 生长激素基因; 聚合酶链反应; ISEW, China, People's Rep., Fujian; large yellow croaker; ); growth trait; growth hormone gene; PCR-single strand conformation polymorphism (PCR-SSCP); single nucleotide polymorphism (SNP)
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-012-1078-y

The growth hormone gene （GH） affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms （SNPs） in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 （G→A） that was negatively correlated with body height and positively correlated with standard length/body height （P≤0.05）. Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 （T→C）. The CD genotype had a significantly positive correlation with both weight and total length （P≤0.01）. These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection.