Application of dispersive liquid–liquid microextraction coupled with vortex-assisted hydrophobic magnetic nanoparticles based solid-phase extraction for determination of aflatoxin M1 in milk samples by sensitive micelle enhanced spectrofluorimetry

• Published in: Talanta (Oxford) Vol. 134; pp. 98 - 104
• Main Authors: Amoli-Diva, Mitra, Taherimaslak, Zohreh, Allahyari, Mehdi, Pourghazi, Kamyar, Manafi, Mohammad Hanif
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2015
• Subjects: Adsorption; Aflatoxin M1; Aflatoxin M1 - analysis; Aflatoxin M1 - chemistry; Animals; Dispersive liquid–liquid microextraction; Environmental Pollutants - analysis; Environmental Pollutants - chemistry; Fluid flow; Food Contamination - analysis; Heptanol - chemistry; Hydrophobic and Hydrophilic Interactions; Hydrophobic magnetic nanoparticles; Joining; Liquid Phase Microextraction; Liquid-liquid extraction; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Micelles; Microscopy, Electron, Transmission; Milk; Milk - chemistry; Nanoparticles; Octoxynol - chemistry; Oleic Acid - chemistry; Solid Phase Extraction; Solvents; Solvents - chemistry; Spectrometry, Fluorescence; Surface-Active Agents - chemistry; Surfactant enhanced spectrofluorimetry; Vortex-assisted dispersive-solid phase extraction; Vortices; Surfactant enhanced spectrofluorimetry; Aflatoxin M1; Vortex-assisted dispersive-solid phase extraction; Hydrophobic magnetic nanoparticles; Dispersive liquid–liquid microextraction
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2014.11.007

An efficient, simple and fast low-density solvent based dispersive liquid–liquid microextraction (LDS-DLLME) followed by vortex-assisted dispersive solid phase extraction (VA-D-SPE) has been developed as a new approach for extraction and preconcentration of aflatoxin M1 in milk samples prior to its micelle enhanced spectrofluorimetic determination. In this LDS-DLLME coupled VA-D-SPE method, milk samples were first treated with methanol/water (80:20, v/v) after removing the fat layer. This solvent was directly used as the dispersing solvent in DLLME along with using 1-heptanol (as a low-density solvent with respect to water) as the extracting solvent. In VA-D-SPE approach, hydrophobic oleic acid modified Fe3O4 nanoparticles were used to retrieve the analyte from the DLLME step. It is considerably that the target of VA-D-SPE was 1-heptanol rather than the aflatoxin M1 directly. The main parameters affecting the efficiency of LDS-DLLME and VA-D-SPE procedures and signal enhancement of aflatoxin M1 were investigated and optimized. Under the optimum conditions, the method was linear in the range from 0.02 to 200µgL−1 with the correlation coefficient (R2) of 0.9989 and detection limit of 13ngL−1. The intra-day precision was 2.9 and 4.3% and the inter-day precision was 2.1 and 3.3% for concentration of 2 and 50µgL−1 respectively. The developed method was applied for extraction and preconcentration of AFM1 in three commercially available milk samples and the results were compared with the official AOAC method. [Display omitted] •High sensitive analysis of AFM1 in milk samples at ngL−1 levels was proposed.•Sample pretreatment was based on DLLME coupled VA-D-SPE procedure.•Determination was based on surfactant enhanced spectrofluorimetric of AFM1.