Chemometrical studies on mixture analysis with a single dynamic microbial sensor

• Published in: Sensors and actuators. B, Chemical Vol. 44; no. 1; pp. 286 - 290
• Main Authors: Slama, Michael, Zaborosch, Christiane, Wienke, Dietrich, Spener, Friedrich
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lausanne Elsevier B.V 01.10.1997; Amsterdam Elsevier; New York, NY
• Subjects: Amperometric sensors; Bacteria; Binary mixtures; Biological and medical sciences; Biosensors; Biotechnology; Cell culture; Cell immobilization; Data reduction; Flow injection analysis; Fundamental and applied biological sciences. Psychology; Least squares approximations; Methods. Procedures. Technologies; Microbial sensor; Multicomponent analysis; Partial least-squares; Various methods and equipments; Flow injection analysis; Multicomponent analysis; Microbial sensor; Partial least-squares; Performance evaluation; Ralstonia eutropha; Oxygen electrode; Microbial electrode; Biosensor; Immobilization; Bacteria; Acetate; Entrapped microorganism; Chemometrics; Flow injection
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/S0925-4005(97)00220-7

A bacterial pure culture of Alclaligenos eutrophus KT02 was immobilized and integrated as a sensor in an amperometrical flow-through system (`dynamic microbial sensor'). The sensor showed characteristic time dependent responses in oxygen consumption for different pure organic analytes. Chemometrical data analysis revealed that the responses are additive and linear concerning single analytes and mixtures. Simultaneous multicomponent analysis of a binary mixture of acetate and gluconate was realized by using only a single dynamic microbial sensor.