Real-time loop-mediated isothermal amplification assay for rapid detection of Fusarium circinatum

is the causal agent of pitch canker, a lethal disease of pine and other conifers. Since is a quarantine organism, its timely detection could efficiently prevent its introduction into new areas or facilitate spread management in already infected sites. In this study, we developed a sequence-specific...

Full description

Saved in:
Bibliographic Details
Published inBioTechniques Vol. 69; no. 1; pp. 11 - 17
Main Authors Stehlíková, Dagmar, Luchi, Nicola, Aglietti, Chiara, Pepori, Alessia Lucia, Diez, Julio Javier, Santini, Alberto
Format Journal Article
LanguageEnglish
Published England Future Science Ltd 01.07.2020
Subjects
elongation factor gene
field-deployable
invasive species
isothermal amplification
LAMP
pine pitch canker
elongation factor gene
LAMP
field-deployable
pine pitch canker
invasive species
isothermal amplification
Online AccessGet full text

Cover

More Information
Summary:is the causal agent of pitch canker, a lethal disease of pine and other conifers. Since is a quarantine organism, its timely detection could efficiently prevent its introduction into new areas or facilitate spread management in already infected sites. In this study, we developed a sequence-specific probe loop-mediated isothermal amplification (LAMP) assay for using a field-deployable portable instrument. The assay was able to recognize the pathogen in host tissues in just 30 min, and the sensitivity of the assay made it possible to detect even small amounts of DNA (as low as 0.5 pg/μl). The high efficiency of this method suggests its use as a standard diagnostic tool during phytosanitary controls.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0736-6205
1940-9818
DOI:10.2144/btn-2019-0168