Wound healing performance of PCL/chitosan based electrospun nanofiber electrosprayed with curcumin loaded chitosan nanoparticles

• Published in: Carbohydrate polymers Vol. 259; p. 117640
• Main Authors: Fahimirad, Shohreh, Abtahi, Hamid, Satei, Parastu, Ghaznavi-Rad, Ehsanollah, Moslehi, Mohsen, Ganji, Ali
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2021
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antioxidants; Antioxidants - chemistry; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Cell Line; cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; cell viability; Chitosan; Chitosan - chemistry; Curcumin; Curcumin - chemistry; Curcumin loaded chitosan nano-encapsulated particles (CURCSNPs); Electrospraying; Electrospun nanofiber; Escherichia coli - drug effects; histology; Humans; Male; methicillin-resistant Staphylococcus aureus; Methicillin-Resistant Staphylococcus aureus - drug effects; Mice; nanofibers; Nanofibers - chemistry; Nanoparticles - chemistry; Nanoparticles - toxicity; Poly(ε-caprolactone); Polyesters - chemistry; Tensile Strength; water vapor; Wound Healing - drug effects; Poly(ε-caprolactone); Electrospun nanofiber; Electrospraying; Curcumin; Curcumin loaded chitosan nano-encapsulated particles (CURCSNPs); Chitosan
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2021.117640

•Curcumin loaded chitosan nano-encapsulated particles (CURCSNPs) were produced successfully.•CURCSNPs were successfully electrosprayed on electrospun PCL/Chitosan/CUR nanofiber.•PCL/Chitosan/CUR electrosprayed with CURCSNPs showed enhanced antibacterial activity.•PCL/Chitosan/CUR electrosprayed with CURCSNPs showed enhanced antioxidant activity.•PCL/Chitosan/CUR electrosprayed with CURCSNPs showed enhanced in vivo wound healing ability. In this study, the electrospun poly(ε-caprolactone) (PCL)/Chitosan (CS)/curcumin (CUR) nanofiber was fabricated successfully with curcumin loaded chitosan nano-encapsulated particles (CURCSNPs). The morphology of the produced CURCSNPs, PCL, PCL/CS, PCL/CS/CUR, and PCL/CS/CUR electrosprayed with CURCSNPs were analyzed by scanning electron microscopy (SEM). The physicochemical properties and biological characteristics of fabricated nanofibers such as antibacterial, antioxidant, cell viability, and in vivo wound healing efficiency and histological assay were tested. The electrospraying of CURCSNPs on surface PCL/CS/CUR nanofiber resulted in the enhanced antibacterial, antioxidant, cell proliferation efficiencies and higher swelling and water vapor transition rates. In vivo examination and Histological analysis showed PCL/CS/CUR electrosprayed with CURCSNPs led to significant improvement of complete well-organized wound healing process in MRSA infected wounds. These results suggest that the application of PCL/CS/CUR electrosprayed with CURCSNPs as a wound dressing significantly facilitates wound healing with notable antibacterial, antioxidant, and cell proliferation properties.