An optimized ultrasound-assisted extraction and simultaneous quantification of 26 characteristic components with four structure types in functional foods from ginkgo seeds

• Published in: Food chemistry Vol. 158; pp. 177 - 185
• Main Authors: Zhou, Guisheng, Yao, Xin, Tang, Yuping, Qian, Dawei, Su, Shulan, Zhang, Li, Jin, Chun, Qin, Yong, Duan, Jin-ao
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.09.2014; Elsevier
• Subjects: Biological and medical sciences; Characteristic constituents; Chromatography, High Pressure Liquid - methods; Food toxicology; Functional Food - analysis; Ginkgo; Ginkgo biloba - chemistry; Medical sciences; Molecular Structure; Principal component analysis; Response surface methodology; Seed; Seeds - chemistry; Tandem Mass Spectrometry - methods; Toxicology; Triple-quadrupole tandem mass spectrometry; Ultrasonic-assisted extraction; Ginkgo; Seed; Characteristic constituents; Ultrasonic-assisted extraction; Response surface methodology; Triple-quadrupole tandem mass spectrometry; Principal component analysis; Seeds; Methodology; Health food; Mass spectrometry MS/MS; Plant origin; Response surface; Extraction; Structure; Ultrasound; Quantitative analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2014.02.116

•Four groups including 26 ginkgo characteristic compounds were simultaneously studied for the first time.•Response surface methodology was used to optimize the process variables.•UHPLC–MS/MS was the first determination of so many components in ginkgo.•The developed method may be applied for other ginkgo products. An optimized method of ultrasound-assisted extraction followed by ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UAE–UHPLC–TQ/MS2) was proposed for the simultaneous extraction and determination of 26 characteristic components covering four structure types (flavonoids, terpene lactones, ginkgolic acids and phenylpropanols) in ginkgo seeds (GSs). The UAE parameters (ultrasound power, time and solvent-to-material ratio) were optimized using a response surface methodology. This is the first report of the simultaneous analysis of 26 compounds in Ginkgo biloba using UHPLC–TQ/MS2; this analysis afforded good linearity, precision, repeatability and accuracy. UAE–UHPLC–TQ/MS2 was successfully applied to ginkgo seed samples, and the analysis showed that GSs are rich in terpene lactones and could be selected as a healthy food resource. The results suggest that UAE–UHPLC–TQ/MS2 might be able to be utilized as a tool for the quality assessment of samples from GSs or other related products using flavonoids, terpene lactones, ginkgolic acids and phenylpropanols as markers.