Comparative UPLC-QTOF-MS-based metabolomics and bioactivities analyses of Garcinia oblongifolia

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1011; pp. 179 - 195
• Main Authors: Li, Ping, AnandhiSenthilkumar, Harini, Wu, Shi-biao, Liu, Bo, Guo, Zhi-yong, Fata, Jimmie E., Kennelly, Edward J., Long, Chun-lin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2016
• Subjects: Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Benzophenones; Benzophenones - chemistry; Benzophenones - pharmacology; Biflavonoids - chemistry; Biflavonoids - pharmacology; Biflavonones; Biocompatibility; Biomedical materials; Breast; Cell Line, Tumor; Cell Survival - drug effects; Chromatography, High Pressure Liquid - methods; Cluster Analysis; Correlation; Cytotoxicity; Fruits; Garcinia - chemistry; Garcinia oblongifolia; Humans; Metabolomics - methods; PCA; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plants (organisms); Principal Component Analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Surgical implants; UPLC-QTOF-MS; Xanthones; Xanthones - chemistry; Xanthones - pharmacology; Antioxidants; Xanthones; UPLC-QTOF-MS; Biflavonones; Benzophenones; Cytotoxicity; Garcinia oblongifolia; PCA
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2015.12.061

[Display omitted] •A strategy for the comparative metabolic profiling and bioactivities of leaf, branch, and fruit extracts of G. oblongifolia is describe.•Forty compounds were identified by UPLC-QTOF-MS and MSE, including 15 compounds reported for the first time from this species.•Heatmap and chemometrics analyses were performed to reveal the distribution and relationship of the chemical constituents among different plant parts. Garcinia oblongifolia Champ. ex Benth. (Clusiaceae) is a well-known medicinal plant from southern China, with edible fruits. However, the phytochemistry and bioactivity of the different plant parts of G. oblongifolia have not been studied extensively. Comparative metabolic profiling and bioactivities of the leaf, branch, and fruit of G. oblongifolia were investigated. A total of 40 compounds such as biflavonoids, xanthones, and benzophenones were identified using UPLC-QTOF-MS and MSE, including 15 compounds reported for the first time from this species. Heatmap analyses found that benzophenones, xanthones, and biflavonoids were predominately found in branches, with benzophenones present in relatively high concentrations in all three plant parts. Xanthones were found to have limited distribution in fruit while biflavonoids were present at only low levels in leaves. In addition, the cytotoxic (MCF-7 breast cancer cell line) and antioxidant (ABTS and DPPH chemical tests) activities of the crude extracts of G. oblongifolia indicate that the branch extract exhibits greater bioactivity than either the leaf or the fruit extracts. Orthogonal partial least squares discriminate analysis was used to find 12 marker compounds, mainly xanthones, from the branches, including well-known antioxidants and cytotoxic agents. These G. oblongifolia results revealed that the variation in metabolite profiles can be correlated to the differences in bioactivity of the three plant parts investigated. This UPLC-QTOF-MS strategy can be useful to identify bioactive constituents expressed differentially in the various plant parts of a single species.