Effects of azotemia on results of the boronate-agarose affinity and ion- exchange methods for glycated hemoglobin

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 30; no. 6; pp. 896 - 898
• Main Authors: Scott, MG, Hoffmann, JW, Meltzer, VN, Siegfried, BA, Chan, KM
• Format: Journal Article
• Language: English
• Published: Washington, DC Am Assoc Clin Chem 01.06.1984; American Association for Clinical Chemistry
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Boronic Acids; Chromatography, Affinity; Chromatography, Ion Exchange; Diabetes Mellitus - blood; False Positive Reactions; Fundamental and applied biological sciences. Psychology; Gels; Glycated Hemoglobin A - analysis; Hemoproteins; Humans; Metalloproteins; Proteins; Sepharose; Uremia - blood; Assay; Biological fluid; Hemoprotein; Affinity chromatography; Coupled method; Clinical biology; Hemoglobin; Glycosylation; Blood; Ion exchange chromatography; Blood plasma
• ISSN: 0009-9147; 1530-8561
• DOI: 10.1093/clinchem/30.6.896

We evaluated the effect of azotemia on results for glycated hemoglobin as measured by a boronate-agarose affinity method and an ion-exchange chromatographic procedure with saline preincubation and found a good correlation. However, values for glycated hemoglobin in samples from nondiabetic patients with various degrees of azotemia were consistently higher with the ion-exchange column procedure (mean, 8.5%) than with the boronate affinity method (mean, 6.2%). The latter method may thus be preferred for monitoring glycated hemoglobin in diabetic patients with impaired renal function.