Screening and quantitative analysis of antioxidants in the fruits of Livistona chinensis R. Br using HPLC-DAD–ESI/MS coupled with pre-column DPPH assay

• Published in: Food chemistry Vol. 135; no. 4; pp. 2802 - 2807
• Main Authors: Yao, Hong, Chen, Yan, Shi, Peiying, Hu, Juan, Li, Shaoguang, Huang, Liying, Lin, Jianhua, Lin, Xinhua
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.12.2012; Elsevier
• Subjects: Antioxidant; Antioxidants - analysis; Arecaceae - chemistry; Biological and medical sciences; Biphenyl Compounds - analysis; C-glycosides; Chromatography, High Pressure Liquid - methods; Flavonoids - analysis; Food industries; Fruit - chemistry; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; HPLC-DAD/ESI-MSn; Livistona chinensis fruits; Picrates - analysis; Plant Extracts - analysis; Pre-column DPPH assay; Spectrometry, Mass, Electrospray Ionization - instrumentation; Spectrometry, Mass, Electrospray Ionization - methods; HPLC-DAD/ESI-MSn; Livistona chinensis fruits; Antioxidant; C-glycosides; Pre-column DPPH assay; Screening; Analysis method; Fruit; HPLC chromatography; Glycoside; HPLC-DAD/ESI-MS; Mass spectrometry; Quantitative analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.07.076

► Six antioxidants were identified in Livistona chinensis fruits for the first time. ► Four flavonoids were found in this plant for the first time. ► Orientin and isoorientin are confirmed to be the main antioxidants in this plant. ► An HPLC method is developed for determination of the six antioxidants in this plant. ► Tricin was found to be the major component in this plant. In this study, a high performance liquid chromatography-photo diode array detection–electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MS) with pre-column DPPH assay is developed for screening the antioxidant components in the fruits of Livistona chinensis R. Br. Accordingly, six antioxidative flavonoids are identified as orientin, isoorientin, vitexin, isovitexin, isorhamnetin-3-O-glucoside and tricin in methanolic extract of L. chinensis fruits, based on their mass spectra and fragmentation patterns. To the best of our knowledge, orientin, isoorientin, isovitexin and isorhamnetin-3-O-glucoside were found firstly in this plant. The free radical scavenging activity of the six antioxidants found is further examined by off-line DPPH assay. The results indicated that the free radical scavenging activity of orientin and isoorientin are stronger than those of two antioxidative drugs, vitamin C and baicalin. In addition, an HPLC-DAD method is firstly established for simultaneous determination of the six antioxidants in L. chinensis fruits. Tricin was found to be the major component in L. chinensis fruits.