Screening and quantitative analysis of antioxidants in the fruits of Livistona chinensis R. Br using HPLC-DAD–ESI/MS coupled with pre-column DPPH assay
► Six antioxidants were identified in Livistona chinensis fruits for the first time. ► Four flavonoids were found in this plant for the first time. ► Orientin and isoorientin are confirmed to be the main antioxidants in this plant. ► An HPLC method is developed for determination of the six antioxida...
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Published in | Food chemistry Vol. 135; no. 4; pp. 2802 - 2807 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Kidlington
Elsevier Ltd
15.12.2012
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | ► Six antioxidants were identified in Livistona chinensis fruits for the first time. ► Four flavonoids were found in this plant for the first time. ► Orientin and isoorientin are confirmed to be the main antioxidants in this plant. ► An HPLC method is developed for determination of the six antioxidants in this plant. ► Tricin was found to be the major component in this plant.
In this study, a high performance liquid chromatography-photo diode array detection–electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MS) with pre-column DPPH assay is developed for screening the antioxidant components in the fruits of Livistona chinensis R. Br. Accordingly, six antioxidative flavonoids are identified as orientin, isoorientin, vitexin, isovitexin, isorhamnetin-3-O-glucoside and tricin in methanolic extract of L. chinensis fruits, based on their mass spectra and fragmentation patterns. To the best of our knowledge, orientin, isoorientin, isovitexin and isorhamnetin-3-O-glucoside were found firstly in this plant. The free radical scavenging activity of the six antioxidants found is further examined by off-line DPPH assay. The results indicated that the free radical scavenging activity of orientin and isoorientin are stronger than those of two antioxidative drugs, vitamin C and baicalin. In addition, an HPLC-DAD method is firstly established for simultaneous determination of the six antioxidants in L. chinensis fruits. Tricin was found to be the major component in L. chinensis fruits. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2012.07.076 |