Specific detection of the novel goose astrovirus using a TaqMan real-time RT-PCR technology

• Published in: Microbial pathogenesis Vol. 137; p. 103766
• Main Authors: Wan, Chunhe, Chen, Cuiteng, Cheng, Longfei, Fu, Guanghua, Shi, Shaohua, Liu, Rongchang, Chen, Hongmei, Fu, Qiuling, Huang, Yu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2019
• Subjects: Animals; Astroviridae Infections - veterinary; Astroviridae Infections - virology; Avastrovirus - classification; Avastrovirus - genetics; Avastrovirus - isolation & purification; Bird Diseases - virology; China; Detection; detection limit; epidemiology; Geese - virology; goslings; Novel goose astrovirus (N-GoAstV); pathogenesis; qRT-PCR; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; Sensitivity and Specificity; TaqMan; Transmission; viruses; China; TaqMan; Detection; Novel goose astrovirus (N-GoAstV); qRT-PCR; Transmission
• ISSN: 0882-4010; 1096-1208; 1096-1208
• DOI: 10.1016/j.micpath.2019.103766

Recently, a novel goose astrovirus (N-GoAstV) was discovered in China, with the transmission route of N-GoAstV unclear. In this study, we developed a TaqMan-based real-time RT-PCR (qRT-PCR) assay for the detection of N-GoAstV infection. After the optimization of the qRT-PCR assay conditions, the results demonstrated that the lower limit of detection for N-GoAstV was 33.4 copies/μL. No cross-reactivity was observed with other goose-origin viruses. Intra-assay and inter-assay variability were ≤1.36% and 2.34%, respectively. N-GoAstV was detected in both field samples, embryos and newly hatched goslings by qRT-PCR assay, provided the view that N-GoAstV may be both horizontally and vertically transmitted. The established qRT-PCR method showed high specificity, sensitivity, and reproducibility, which can be used in future investigations on the pathogenesis and epidemiology of N-GoAstV. •We established a TaqMan real-time RT-PCR method for detection of novel goose astrovirus (N-GoAstV).•The method showed high sensitivity, specificity and reproducibility for detection of N-GoAstV.•This study provided the view that N-GoAstV may be possible vertically transmitted.