Relationship between calcium permeability and rectification properties of AMPA receptors in cultured rat hippocampal neurons

• Published in: Neuroscience letters Vol. 173; no. 1; pp. 14 - 16
• Main Authors: Iino, Masae, Mochizuki, Shinobu, Ozawa, Seiji
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 23.05.1994; Elsevier
• Subjects: AMPA subtype; Animals; Biological and medical sciences; Ca 2+ permeability; Calcium - metabolism; Cells, Cultured; Fundamental and applied biological sciences. Psychology; Glutamate receptor; Hippocampal neuron; Hippocampus - cytology; Hippocampus - metabolism; Hippocampus - physiology; Isolated neuron and nerve. Neuroglia; I–V relation; Kainate; Kainic Acid - pharmacology; Membrane Potentials - drug effects; Membrane Potentials - physiology; Neurons - drug effects; Neurons - metabolism; Neurons - physiology; Rats; Receptors, AMPA - drug effects; Receptors, AMPA - metabolism; Receptors, AMPA - physiology; Vertebrates: nervous system and sense organs; Glutamate receptor; AMPA subtype; Kainate; Hippocampal neuron; Ca 2+ permeability; I–V relation; Calcium; Rat; Rodentia; Central nervous system; Electrophysiology; Ion transport; Ionic current; In vitro; Vertebrata; AMPA receptor; Mammalia; Neuron; Neurotransmitter; Excitatory aminoacid; Hippocampus; Brain (vertebrata)
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/0304-3940(94)90139-2

Cultured rat hippocampal neurons were classified into three groups on the basis of the functional properties of their AMPA-subtype glutamate receptors. The type I neuron had AMPA receptors with an outwardly rectifying I–V relation and little permeability to Ca 2+ whereas the AMPA receptors in the type II neuron were characterized by marked inward rectification and high Ca 2+ permeability. In the third type of neuron, the responses of AMPA receptors exhibited intermediate properties in both I–V relation and Ca 2+ permeability. We suggest that these intermediate properties in the third type of neuron reflect the coexistence of Ca 2+-permeant and Ca 2+-impermeant AMPA receptors.